PDF(Pubmed)
Abstract：
Rotavirus A (RVA) causes gastroenteritis in humans and animals. The zoonotic potential of RVA has been reported and raises major concerns, especially in animal-human interface settings. The study aimed to characterize and investigate the genetic diversity among RVAs in dogs and cats in Thailand. We collected 572 rectal swab samples from dogs and cats in Bangkok animal hospitals from January 2020 to June 2021. The one-step RT-PCR assay detected RVAs in 1.92% (11/572) of the samples, with 2.75% (8/290) in dogs and 1.06% (3/282) in cats. Two canine RVA and one feline RVA were subjected to whole genome sequencing. Our results showed that all three viruses were identified as RVA genotype G3P[3]. The genetic constellation of RVAs is unique for different species. For canine RVAs is G3-P [3]-I3-R3-C3-M3-A9-N2-T3-E3-H6, while Feline RVA is G3-P [3]-I8-R3-C3-M3-A9-N3-T3-E3-H6. Notably, both canine and feline RVAs contained the AU-1 genetic constellation with multiple reassortments. The results of phylogenetic, genetic, and bootscan analyses showed that canine RVAs may have reassorted from dog, human, and cat RVAs. While feline RVA was closely related to RVAs in humans, bats, and simians. This study provided genetic characteristics and diversity of RVAs in dogs and cats and suggested possible multiple reassortments, suggesting the zoonotic potential of the viruses. Thus, public health awareness should be raised regarding the zoonotic potential of RVAs in dogs and cats. Further studies on RVAs on a larger scale in dogs and cats in Thailand are needed.
摘要：
轮状病毒A(RVA)在人和动物中引起胃肠炎。已经报道了RVA的人畜共患潜力，并引起了主要关注，尤其是在动物-人类界面设置中。该研究旨在表征和调查泰国狗和猫的RVA之间的遗传多样性。我们从2020年1月至2021年6月在曼谷动物医院收集了572只来自狗和猫的直肠拭子样本。一步法RT-PCR在1.92%(11/572)的样品中检测到RVA,狗占2.75%(8/290)，猫占1.06%(3/282)。对两个犬RVA和一个猫RVA进行全基因组测序。我们的结果表明，所有三种病毒都被鉴定为RVA基因型G3P[3]。RVA的遗传星座对于不同物种是独特的。对于犬RVA,RVA是G3-P[3]-R3-C3-M3-A9-N2-T3-E3-H6,而猫RVA是G3-P[3]-I8-R3-C3-M3-A9-N3-T3-E3-H6。值得注意的是,犬和猫的RVA都包含AU-1遗传星座，并进行了多次重组。系统发育的结果，遗传,Bootscan分析显示，犬RVA可能是从狗身上重新分类的，人类,和猫RVAs。虽然猫RVA与人类的RVA密切相关，蝙蝠,和猿猴。这项研究提供了狗和猫的RVA的遗传特征和多样性，并提出了可能的多种重排，这表明了病毒的人畜共患潜力。因此，关于狗和猫的RVA的人畜共患潜力，应提高公众健康意识。需要在泰国的狗和猫中对RVA进行更大规模的进一步研究。
