Abstract：
Glanders, a highly contagious and often fatal disease affecting equids, is caused by Burkholderia mallei. Although sporadic cases of equine glanders have recently been documented in Mongolia, genome sequencing and molecular studies of the bacteria within this region are lacking. This study provided the first molecular characterization of B. mallei isolated from four native Mongolian horses from two different provinces in 2019 and 2022 by applying whole-genome sequencing with two SNP types (previously developed genotyping with 15 SNP markers that provide global coverage of the B. mallei population and the core genome coding SNP typing developed in this study). The Mongolian isolates were located within the L3B1 cluster, which was previously associated with the V-120 strain from Russia. Within the L3B1 cluster shared by neighboring countries, they were in a unique subbranch. In this study, specific SNP markers unique to the Mongolian strains were identified to track these strains using a high-resolution melting analysis (HRMA). This study revealed the unique phylogenetic background of Mongolian strains isolated from the eastern part of Mongolia. HRMA specific to the Mongolian subbranch may contribute to the molecular epidemiological monitoring of glanders in Mongolia and surrounding countries.
摘要：
格兰德斯,一种高度传染性且通常致命的疾病，是由BurkholderiaMallei引起的.尽管最近在蒙古有零星的马腺体病例记录，缺乏该地区细菌的基因组测序和分子研究。这项研究通过应用两种SNP类型的全基因组测序（先前开发的具有15个SNP标记的基因分型技术，提供了2019年和2022年从来自两个不同省份的四匹本地蒙古马分离的B.mallei的第一个分子表征。蒙古分离株位于L3B1簇内，以前与来自俄罗斯的V-120毒株有关。在邻国共享的L3B1集群内，他们在一个独特的分支。在这项研究中,使用高分辨率熔解分析(HRMA)鉴定了蒙古菌株特有的特定SNP标记以追踪这些菌株。这项研究揭示了从蒙古东部分离出的蒙古菌株的独特系统发育背景。蒙古分支机构特有的HRMA可能有助于蒙古及周边国家的腺体分子流行病学监测。
