Abstract：
A widely applicable original gas chromatography-tandem mass spectrometry (GC-MS/MS) method was explored to qualitatively and quantitatively measure enrofloxacin and ofloxacin residues in chicken tissues and pork. The experimental samples were processed based on liquid-liquid extraction (LLE) and solid-phase extraction (SPE). Trimethylsilyl diazomethane (TMSD) was chosen to react derivatively with enrofloxacin and ofloxacin. In total, 78.25% ∼ 90.56% enrofloxacin and 78.43% ∼ 91.86% ofloxacin was recovered from the blank fortified samples. The limits of detection (LODs) were 0.7-1.0 μg/kg and 0.1-0.2 μg/kg, respectively. The limits of quantitation (LOQs) were 1.6-1.9 μg/kg and 0.3-0.4 μg/kg, respectively. It was verified that various experimental data met the requirements of the FAO & WHO (2014) for the detection of veterinary drug residues. Real samples obtained from local markets were analysed using the established method, and no residues of enrofloxacin or ofloxacin were detected in the samples.
摘要：
探索了一种广泛适用的原始气相色谱-串联质谱（GC-MS/MS）方法，用于定性和定量测定鸡肉组织和猪肉中恩诺沙星和氧氟沙星的残留。采用液液萃取(LLE)和固相萃取(SPE)对实验样品进行处理。选择三甲基甲硅烷基重氮甲烷（TMSD）与恩诺沙星和氧氟沙星进行衍生反应。总的来说,从空白强化样品中回收了78.25%~90.56%的恩诺沙星和78.43%~91.86%的氧氟沙星。检出限(LODs)分别为0.7-1.0μg/kg和0.1-0.2μg/kg,分别。定量限（LOQs）为1.6-1.9μg/kg和0.3-0.4μg/kg，分别。经验证,各项实验数据均符合FAO&WHO(2014)关于兽药残留检测的要求。使用既定的方法分析了从当地市场获得的真实样本，样品中未检测到恩诺沙星和氧氟沙星的残留。
