Abstract：
The utilization of biomimetic membranes supported by advanced self-assembled monolayers is gaining attraction as a promising sensing tool. Biomimetic membranes offer exceptional biocompatibility and adsorption capacity upon degradation, transcending their role as mere research instruments to open new avenues in biosensing. This study focused on anchoring a sparsely tethered bilayer lipid membrane onto a self-assembled monolayer composed of a biodegradable polymer, functionalized with poly(ethylene glycol)-cholesterol moieties, for lipid membrane integration. Real-time monitoring via quartz crystal microbalance, coupled with characterization using surface-enhanced infrared absorption spectroscopy and electrochemical impedance spectroscopy, provided comprehensive insights into each manufacturing phase. The resulting lipid layer, along with transmembrane pores formed by gramicidin A, exhibited robust stability. Electrochemical impedance spectroscopy analysis confirmed membrane integrity, successful pore formation, and consistent channel density. Notably, gramicidin A demonstrated sustained functionality as an ion channel upon reconstitution, with its functionality being effectively blocked and inhibited in the presence of calcium ions. These findings mark significant strides in developing intricate biodegradable nanomaterials with promising applications in biomedicine.
摘要：
利用由先进的自组装单层支持的仿生膜作为一种有前途的传感工具正在获得吸引力。仿生膜在降解时提供卓越的生物相容性和吸附能力，超越了他们作为单纯研究工具的角色，为生物传感开辟了新的途径。这项研究的重点是将稀疏束缚的双层脂质膜锚定到由生物可降解聚合物组成的自组装单层上，用聚乙二醇-胆固醇部分官能化，用于脂质膜整合。通过石英晶体微天平实时监控，结合使用表面增强红外吸收光谱和电化学阻抗谱的表征，提供了对每个制造阶段的全面见解。产生的脂质层，以及由小草菌素A形成的跨膜孔，表现出稳健的稳定性。电化学阻抗谱分析证实了膜的完整性，成功的孔隙形成，和一致的通道密度。值得注意的是,在重组时，球藻素A表现出作为离子通道的持续功能，在钙离子的存在下，其功能被有效阻断和抑制。这些发现标志着在开发复杂的可生物降解纳米材料方面取得了重大进展，并在生物医学中具有广阔的应用前景。
