Abstract：
Porcine rotavirus (PoRV) is one of the main pathogens causing diarrhea in piglets, and multiple genotypes coexist. However, an effective vaccine is currently lacking. Here, the potential adjuvant of nonstructural protein 4 (NSP4) and highly immunogenic structural protein VP4 prompted us to construct recombinant NSP486-175aa (NSP4*) and VP426-476aa (VP4*) proteins, combine them as immunogens to evaluate their efficacy. Results indicated that NSP4* enhanced systemic and local mucosal responses induced by VP4*. The VP4*-IgG, VP4*-IgA in feces and IgA-secreting cells in intestines induced by the co-immunization were significantly higher than those induced by VP4* alone. Co-immunization of NSP4* and VP4* also induced strong cellular immunity with significantly increased IFN-λ than the single VP4*. Summarily, the NSP4* as a synergistical antigen exerted limited effects on the PoRV NAbs elevation, but conferred strong VP4*-specific mucosal and cellular efficacy, which lays the foundation for the development of a more effective porcine rotavirus subunit vaccine.
摘要：
猪轮状病毒(PoRV)是引起仔猪腹泻的主要病原之一,多种基因型共存。然而,目前缺乏有效的疫苗。这里,非结构蛋白4（NSP4）和高免疫原性结构蛋白VP4的潜在佐剂促使我们构建重组NSP486-175aa（NSP4*）和VP426-476aa（VP4*）蛋白，将它们作为免疫原来评估它们的功效。结果表明，NSP4*增强了VP4*诱导的全身和局部粘膜反应。VP4*-IgG，共同免疫诱导的粪便中的VP4*-IgA和肠道中的IgA分泌细胞显著高于单独的VP4*诱导的那些。NSP4*和VP4*的共同免疫还诱导强细胞免疫,其IFN-λ比单个VP4*显著增加。总之,NSP4*作为协同抗原对PoRVNAb升高产生有限的影响，但赋予了强大的VP4*特异性粘膜和细胞功效，为开发更有效的猪轮状病毒亚单位疫苗奠定了基础。
