%0 Journal Article
%T The synergy of recombinant NSP4 and VP4 from porcine rotavirus elicited a strong mucosal response.
%A Li S
%A Tang X
%A Zhou J
%A Bian X
%A Wang J
%A Gu L
%A Zhu X
%A Tao R
%A Sun M
%A Zhang X
%A Li B
%J Virology
%V 597
%N 0
%D 2024 Jun 4
%M 38850894
%F 3.513
%R 10.1016/j.virol.2024.110130
%X Porcine rotavirus (PoRV) is one of the main pathogens causing diarrhea in piglets, and multiple genotypes coexist. However, an effective vaccine is currently lacking. Here, the potential adjuvant of nonstructural protein 4 (NSP4) and highly immunogenic structural protein VP4 prompted us to construct recombinant NSP486-175aa (NSP4*) and VP426-476aa (VP4*) proteins, combine them as immunogens to evaluate their efficacy. Results indicated that NSP4* enhanced systemic and local mucosal responses induced by VP4*. The VP4*-IgG, VP4*-IgA in feces and IgA-secreting cells in intestines induced by the co-immunization were significantly higher than those induced by VP4* alone. Co-immunization of NSP4* and VP4* also induced strong cellular immunity with significantly increased IFN-λ than the single VP4*. Summarily, the NSP4* as a synergistical antigen exerted limited effects on the PoRV NAbs elevation, but conferred strong VP4*-specific mucosal and cellular efficacy, which lays the foundation for the development of a more effective porcine rotavirus subunit vaccine.