PDF(Pubmed)
Abstract：
Intralesional corticosteroid injections are a first-line treatment for keloids; yet clinical treatment results are highly variable and often suboptimal. Variation in triamcinolone acetonide (TAC) biodistribution may be an important reason for the variable effects of TAC treatment in keloids. In this exploratory study we investigated the biodistribution of TAC in keloids and normal skin using different drug delivery techniques. Fluorescent-labeled TAC suspension was administered into keloids and normal skin with a hypodermic needle and an electronic pneumatic jet injector. TAC biodistribution was represented by the fluorescent TAC volume and 3D biodistribution shape of TAC, using a 3D-Fluorescence-Imaging Cryomicrotome System. Twenty-one keloid and nine normal skin samples were analyzed. With needle injections, the mean fluorescent TAC volumes were 990 µl ± 479 in keloids and 872 µl ± 227 in normal skin. With the jet injector, the mean fluorescent TAC volumes were 401 µl ± 252 in keloids and 249 µl ± 67 in normal skin. 3D biodistribution shapes of TAC were highly variable in keloids and normal skin. In conclusion, TAC biodistribution in keloids is highly variable for both needle and jet injection. This may partly explain the variable treatment effects of intralesional TAC in keloids. Future research is needed to confirm this preliminary finding and to optimize drug delivery in keloids.
摘要：
病灶内注射皮质类固醇是瘢痕疙瘩的一线治疗方法；然而，临床治疗结果变化很大，而且往往不够理想。曲安奈德（TAC）生物分布的变化可能是TAC治疗瘢痕疙瘩效果变化的重要原因。在这项探索性研究中，我们使用不同的药物递送技术研究了TAC在瘢痕疙瘩和正常皮肤中的生物分布。用皮下注射针和电子气动喷射注射器将荧光标记的TAC悬浮液施用于瘢痕疙瘩和正常皮肤。TAC生物分布由TAC的荧光体积和3D生物分布形状表示，使用3D荧光成像低温切片机系统。分析了21个瘢痕疙瘩和9个正常皮肤样本。针头注射，瘢痕疙瘩的平均荧光TAC体积为990μl±479,正常皮肤为872μl±227.有了喷射喷射器，瘢痕疙瘩的平均荧光TAC体积为401μl±252,正常皮肤为249μl±67.TAC的3D生物分布形状在瘢痕疙瘩和正常皮肤中高度可变。总之,对于针头注射和喷射注射,瘢痕疙瘩中的TAC生物分布是高度可变的。这可能部分解释了病灶内TAC在瘢痕疙瘩中的可变治疗效果。需要未来的研究来证实这一初步发现，并优化瘢痕疙瘩的药物输送。
