Abstract：
Two alkaliphilic, Gram-stain-negative bacterial strains (MEB004T and MEB108T) were isolated from water samples collected from Lonar lake, India. The phylogenetic analysis of their 16S rRNA gene sequences showed the highest similarity to A. delamerensis DSM 18314T (98.4%), followed by A. amylolytica DSM 18337T and A. collagenimarina JCM 14267T (97.9%). The genome sizes of strains MEB004T and MEB108T were determined to be 3,858,702 and 4,029,814 bp, respectively, with genomic DNA G + C contents of 51.4 and 51.9%. Average Nucleotide Identity, DNA-DNA Hybridization and Amino Acid Identity values between strains (MEB004T and MEB108T) and A. amylolytica DSM 18337T were (82.3 and 85.5), (25.0 and 29.2) and (86.7 and 90.2%). Both novel strains produced industrially important enzymes, such as amylase, lipase, cellulase, caseinase, and chitinase at pH 10 evidenced by the genomic presence of carbohydrate-active enzymes encoding genes. Genomic analyses further identified pH tolerance genes, affirming their adaptation to alkaline Lonar Lake. Dominant fatty acids were Summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), C16:0, Summed feature 3, Sum In Feature 2 and C12:0 3OH. The prevalent polar lipids included phosphatidyl ethanolamine, phosphatidyl glycerol, and diphosphatidyl glycerol. The major respiratory quinone was ubiquinone-8. Based on the polyphasic data, we propose the classification of strains MEB004T and MEB108T as novel species within the genus Alkalimonas assigning the names Alkalimonas mucilaginosa sp. nov. and Alkalimonas cellulosilytica sp. nov., respectively. The type strains are MEB004T (= MCC 5208T = JCM 35954T = NCIMB 15460T) and MEB108T (= MCC 5330T = JCM 35955T = NCIMB 15461T).
摘要：
两个嗜碱性，从Lonar湖收集的水样中分离出革兰氏染色阴性细菌菌株（MEB004T和MEB108T），印度。对其16SrRNA基因序列的系统发育分析表明，与A.delamerensisDSM18314T的相似性最高（98.4％），其次是A.amylolyticaDSM18337T和A.colunimimarinaJCM14267T（97.9%）。菌株MEB004T和MEB108T的基因组大小分别为3,858,702和4,029,814bp，分别,基因组DNAG+C含量分别为51.4%和51.9%。平均核苷酸同一性，菌株（MEB004T和MEB108T）和溶淀粉A.DSM18337T之间的DNA-DNA杂交和氨基酸同一性值分别为（82.3和85.5），（25.0和29.2）和（86.7和90.2%）。两种新菌株都产生了工业上重要的酶，如淀粉酶，脂肪酶,纤维素酶,酪蛋白蛋白酶,和pH10的几丁质酶由基因组中存在的碳水化合物活性酶编码基因证明。基因组分析进一步确定了pH耐受性基因，肯定他们对碱性Lonar湖的适应。显性脂肪酸被总结为特征8(C18:1ω7c和/或C18:1ω6c)，C16:0,求和特征3,总和特征2和C12:03OH。普遍的极性脂质包括磷脂酰乙醇胺，磷脂酰甘油，和二磷脂酰甘油。主要的呼吸醌是泛醌-8。根据多相数据，我们建议将菌株MEB004T和MEB108T分类为Alkalimonas属中的新物种，将名称命名为Alkalimonasmusiilaginosasp。11月。和碱性单胞菌。11月。,分别。菌株类型为MEB004T(=MCC5208T=JCM35954T=NCIMB15460T)和MEB108T(=MCC5330T=JCM35955T=NCIMB15461T)。
