Abstract：
In this research, to provide an optimal growth medium for the production of iturin A, the concentrations of key amino acid precursors were optimized in shake flask cultures using the response surface method. The optimized medium were applied in a biofilm reactor for batch fermentation, resulting in enhanced production of iturin A. On this basis, a step-wise pH control strategy and a combined step-wise pH and temperature control strategy were introduced to further improve the production of iturin A. Finally, the fed-batch fermentation was performed based on combined step-wise pH and temperature control. The titer and productivity of iturin A reached 7.86 ± 0.23 g/L and 65.50 ± 1.92 mg/L/h, respectively, which were 37.65 and 65.20% higher than that before process optimization.
摘要：
在这项研究中,为生产iturinA提供最佳的生长培养基，用响应面法优化摇瓶培养中关键氨基酸前体的浓度。将优化的培养基应用于生物膜反应器中进行分批发酵，从而提高了iturinA的产量。在此基础上，引入了逐步pH控制策略以及逐步pH和温度的组合控制策略，以进一步提高iturinA的产量。最后，基于组合的逐步pH和温度控制进行补料分批发酵。伊杜林A的效价和产率分别达到7.86±0.23g/L和65.50±1.92mg/L/h,分别,分别比工艺优化前高出37.65和65.20%。
