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Abstract：
To investigate the expression of Inhibin B between various clinical stages, Chinese medicine dialectic typing, and in nasopharyngeal carcinoma (NPC) tissues and serum, and to evaluate the potential of Inhibin B as a new biomarker for NPC. Paraffin specimens of pathologically confirmed NPC tissues and paracancerous tissues were retrospectively collected, and the expression of Inhibin α (INHA) and Inhibin βB (INHBB) was detected by SP method, and their relationship with clinicopathological indexes was analyzed; in addition, patients with NPC who had received radiotherapy were included as the study subjects, and Epstein-Barr virus DNA (EBV-DNA), INHA, and INHBB in patients were detected by using the fluorescence quantitative polymerase chain reaction, enzyme-linked immunosorbent assay, and chemiluminescent immuno-sandwiching method, respectively. EBV-DNA, EBV-viral capsid antigen-immunoglobulin A (VCA IgA), INHA, and INHBB were detected in the patients, respectively, and their relationships with traditional Chinese medicine (TCM) patterns were also analyzed. The expression of INHA and INHBB in NPC tissues was lower than that in paracancerous tissues, and the expression of INHA in NPC patients was correlated with lymphatic metastasis, clinical staging, and TCM staging; the levels of EBV-DNA and VCA IgA were higher than that of healthy populations in NPC patients and were higher than that of patients with stage III + IV than that of patients with stage I + II, and the levels of INHA and INHBB were lower than those of healthy populations and were lower than those of patients with stage III + IV than that of patients with stage I + II. The levels of INHA and INHBB in nasopharyngeal cancer patients were lower than those in healthy people, and the levels in stage III + IV patients were lower than those in stage I + II patients. The levels of EBV-DNA and VCA IgA in nasopharyngeal cancer patients were correlated with the Chinese medicine patterns, and had different patterns. The expression of Inhibin B may be related to the progression of NPC, and it has certain typing significance for different TCM syndromes of NPC, which is helpful for TCM typing diagnosis.
摘要：
探讨抑制素B在不同临床分期间的表达,中医辨证分型,在鼻咽癌（NPC）组织和血清中，并评估抑制素B作为NPC新生物标志物的潜力。回顾性收集经病理证实的NPC组织和癌旁组织的石蜡标本，SP法检测抑制素α(INHA)和抑制素βB(INHBB)的表达,并分析了它们与临床病理指标的关系；此外，接受放疗的NPC患者被纳入研究对象，和EB病毒DNA（EBV-DNA），INHA,用荧光定量聚合酶链反应检测患者的INHBB,酶联免疫吸附测定，化学发光免疫夹心法，分别。EBV-DNA,EBV病毒衣壳抗原免疫球蛋白A（VCAIgA），INHA,在患者中检测到INHBB，分别,并分析了它们与中医模式的关系。鼻咽癌组织中INHA和INHBB的表达低于癌旁组织，INHA在鼻咽癌患者中的表达与淋巴结转移有关，临床分期,和中医分期;鼻咽癌患者EBV-DNA和VCAIgA水平均高于健康人群,且Ⅲ+Ⅳ期患者高于Ⅰ+Ⅱ期患者,INHA和INHBB水平低于健康人群，低于III+IV期患者，低于I+II期患者。鼻咽癌患者的INHA和INHBB水平低于健康人，III+IV期患者的水平低于I+II期患者。鼻咽癌患者EBV-DNA和VCAIgA水平与中医证型相关,有不同的模式。抑制素B的表达可能与鼻咽癌的进展有关。对鼻咽癌不同中医证型具有一定的分型意义,有助于中医分型诊断。
