PDF(Pubmed)
Abstract：
UNASSIGNED: Gliomas constitute a category of malignant tumors originating from brain tissue, representing the majority of intracranial malignancies. Previous research has demonstrated the pivotal role of CLEC7A in the progression of various cancers, yet its specific implications within gliomas remain elusive. The primary objective of this study was to investigate the prognostic significance and immune therapeutic potential of CLEC7A in gliomas through the integration of bioinformatics and clinical pathological analyses.
UNASSIGNED: This investigation involved examining and validating the relationship between CLEC7A and glioma using samples from Hospital, along with data from TCGA, GEO, GTEx, and CGGA datasets. Subsequently, we explored its prognostic value, biological functions, expression location, and impact on immune cells within gliomas. Finally, we investigated its potential impact on the chemotaxis and polarization of macrophages.
UNASSIGNED: The expression of CLEC7A is upregulated in gliomas, and its levels escalate with the malignancy of tumors, establishing it as an independent prognostic factor. Functional enrichment analysis revealed a significant correlation between CLEC7A and immune function. Subsequent examination of immune cell differential expression demonstrated a robust association between CLEC7A and M2 macrophages. This conclusion was further substantiated through single-cell analysis, immunofluorescence, and correlation studies. Finally, the knockout of CLEC7A in M2 macrophages resulted in a noteworthy reduction in macrophage chemotaxis and polarization factors.
UNASSIGNED: CLEC7A expression is intricately linked to the pathology and molecular characteristics of gliomas, establishing its role as an independent prognostic factor for gliomas and influencing macrophage function. It could be a promising target for immunotherapy in gliomas.
UNASSIGNED: This investigation involved examining and validating the relationship between CLEC7A and glioma using samples from Hospital, along with data from TCGA, GEO, GTEx, and CGGA datasets. Subsequently, we explored its prognostic value, biological functions, expression location, and impact on immune cells within gliomas. Finally, we investigated its potential impact on the chemotaxis and polarization of macrophages.
UNASSIGNED: The expression of CLEC7A is upregulated in gliomas, and its levels escalate with the malignancy of tumors, establishing it as an independent prognostic factor. Functional enrichment analysis revealed a significant correlation between CLEC7A and immune function. Subsequent examination of immune cell differential expression demonstrated a robust association between CLEC7A and M2 macrophages. This conclusion was further substantiated through single-cell analysis, immunofluorescence, and correlation studies. Finally, the knockout of CLEC7A in M2 macrophages resulted in a noteworthy reduction in macrophage chemotaxis and polarization factors.
UNASSIGNED: CLEC7A expression is intricately linked to the pathology and molecular characteristics of gliomas, establishing its role as an independent prognostic factor for gliomas and influencing macrophage function. It could be a promising target for immunotherapy in gliomas.
摘要：
■胶质瘤是一种起源于脑组织的恶性肿瘤，代表大多数颅内恶性肿瘤。先前的研究已经证明了CLEC7A在各种癌症进展中的关键作用，然而，其在神经胶质瘤中的具体含义仍然难以捉摸。本研究的主要目的是通过整合生物信息学和临床病理分析，探讨CLEC7A在神经胶质瘤中的预后意义和免疫治疗潜力。
■这项调查涉及使用医院的样本检查和验证CLEC7A与神经胶质瘤之间的关系，以及来自TCGA的数据，GEO,GTEx,和CGGA数据集。随后，我们探讨了它的预后价值，生物学功能,表达式位置,以及对神经胶质瘤内免疫细胞的影响。最后,我们研究了其对巨噬细胞趋化和极化的潜在影响.
■CLEC7A的表达在胶质瘤中上调,它的水平随着肿瘤的恶性而上升，将其确立为独立的预后因素。功能富集分析显示CLEC7A与免疫功能之间存在显着相关性。随后对免疫细胞差异表达的检查证明了CLEC7A和M2巨噬细胞之间的强烈关联。通过单细胞分析进一步证实了这一结论,免疫荧光，和相关性研究。最后,在M2巨噬细胞中敲除CLEC7A导致巨噬细胞趋化性和极化因子显著减少.
■CLEC7A的表达与胶质瘤的病理和分子特征密切相关，确立其作为胶质瘤和影响巨噬细胞功能的独立预后因素的作用。它可能是神经胶质瘤免疫治疗的一个有希望的靶点。
■这项调查涉及使用医院的样本检查和验证CLEC7A与神经胶质瘤之间的关系，以及来自TCGA的数据，GEO,GTEx,和CGGA数据集。随后，我们探讨了它的预后价值，生物学功能,表达式位置,以及对神经胶质瘤内免疫细胞的影响。最后,我们研究了其对巨噬细胞趋化和极化的潜在影响.
■CLEC7A的表达在胶质瘤中上调,它的水平随着肿瘤的恶性而上升，将其确立为独立的预后因素。功能富集分析显示CLEC7A与免疫功能之间存在显着相关性。随后对免疫细胞差异表达的检查证明了CLEC7A和M2巨噬细胞之间的强烈关联。通过单细胞分析进一步证实了这一结论,免疫荧光，和相关性研究。最后,在M2巨噬细胞中敲除CLEC7A导致巨噬细胞趋化性和极化因子显著减少.
■CLEC7A的表达与胶质瘤的病理和分子特征密切相关，确立其作为胶质瘤和影响巨噬细胞功能的独立预后因素的作用。它可能是神经胶质瘤免疫治疗的一个有希望的靶点。
