PDF(Pubmed)
Abstract：
UNASSIGNED: Pseudomonas aeruginosa is notorious for its multidrug resistance and its involvement in hospital-acquired infections. In this study, 20 bacterial strains isolated from soil samples near the Hindan River in Ghaziabad, India, were investigated for their biochemical and morphological characteristics, with a focus on identifying strains with exceptional drug resistance and pyocyanin production.
UNASSIGNED: The isolated bacterial strains were subjected to biochemical and morphological analyses to characterize their properties, with a particular emphasis on exopolysaccharide production. Strain GZB16/CEES1, exhibiting remarkable drug resistance and pyocyanin production. Biochemical and molecular analyses, including sequencing of its 16S rRNA gene (accession number LN735036.1), plasmid-curing assays, and estimation of plasmid size, were conducted to elucidate its drug resistance mechanisms and further pyocynin based target the Candida albicans Strain GZB16/CEES1 demonstrated 100% resistance to various antibiotics used in the investigation, with plasmid-curing assays, suggesting plasmid-based resistance gene transmission. The plasmid in GZB16/CEES1 was estimated to be approximately 24 kb in size. The study focused on P. aeruginosa\'s pyocyanin production, revealing its association with anticandidal activity. The minimum inhibitory concentration (MIC) of the bacterial extract against Candida albicans was 50 μg/ml, with a slightly lower pyocyanin-based MIC of 38.5 μg/ml. Scanning electron microscopy illustrated direct interactions between P. aeruginosa strains and Candida albicans cells, leading to the destruction of the latter.
UNASSIGNED: These findings underscore the potential of P. aeruginosa in understanding microbial interactions and developing strategies to combat fungal infections. The study highlights the importance of investigating bacterial-fungal interactions and the role of pyocyanin in antimicrobial activity. Further research in this area could lead to the development of novel therapeutic approaches for combating multidrug-resistant infections.
UNASSIGNED: The isolated bacterial strains were subjected to biochemical and morphological analyses to characterize their properties, with a particular emphasis on exopolysaccharide production. Strain GZB16/CEES1, exhibiting remarkable drug resistance and pyocyanin production. Biochemical and molecular analyses, including sequencing of its 16S rRNA gene (accession number LN735036.1), plasmid-curing assays, and estimation of plasmid size, were conducted to elucidate its drug resistance mechanisms and further pyocynin based target the Candida albicans Strain GZB16/CEES1 demonstrated 100% resistance to various antibiotics used in the investigation, with plasmid-curing assays, suggesting plasmid-based resistance gene transmission. The plasmid in GZB16/CEES1 was estimated to be approximately 24 kb in size. The study focused on P. aeruginosa\'s pyocyanin production, revealing its association with anticandidal activity. The minimum inhibitory concentration (MIC) of the bacterial extract against Candida albicans was 50 μg/ml, with a slightly lower pyocyanin-based MIC of 38.5 μg/ml. Scanning electron microscopy illustrated direct interactions between P. aeruginosa strains and Candida albicans cells, leading to the destruction of the latter.
UNASSIGNED: These findings underscore the potential of P. aeruginosa in understanding microbial interactions and developing strategies to combat fungal infections. The study highlights the importance of investigating bacterial-fungal interactions and the role of pyocyanin in antimicrobial activity. Further research in this area could lead to the development of novel therapeutic approaches for combating multidrug-resistant infections.
摘要：
■铜绿假单胞菌因其多重耐药性和参与医院获得性感染而臭名昭著。在这项研究中,从加济阿巴德印度河附近的土壤样本中分离出20种细菌菌株，印度,对其生化和形态特征进行了研究，重点是鉴定具有特殊耐药性和产卵青素的菌株。
■对分离的细菌菌株进行生化和形态学分析，以表征其特性，特别强调胞外多糖的生产。菌株GZB16/CEES1，表现出显著的耐药性和产卵氰素。生化和分子分析，包括其16SrRNA基因的测序（登录号LN735036.1），质粒固化试验，和质粒大小的估计，进行了阐明其耐药机制和进一步基于pyocyin的目标，白色念珠菌菌株GZB16/CEES1对研究中使用的各种抗生素表现出100％的耐药性，用质粒固化试验，表明基于质粒的抗性基因传递。估计GZB16/CEES1中的质粒大小约为24kb。本研究的重点是铜绿假单胞菌的绿脓苷生产，揭示了它与抗虫活性的关联。细菌提取物对白色念珠菌的最低抑菌浓度（MIC）为50μg/ml，具有38.5μg/ml的略低的基于绿脓苷的MIC。扫描电子显微镜显示铜绿假单胞菌菌株和白色念珠菌细胞之间的直接相互作用，导致后者的毁灭。
■这些发现强调了铜绿假单胞菌在理解微生物相互作用和制定防治真菌感染策略方面的潜力。该研究强调了研究细菌-真菌相互作用以及绿脓苷在抗菌活性中的作用的重要性。在这一领域的进一步研究可能导致开发新的治疗方法来对抗多药耐药感染。
■对分离的细菌菌株进行生化和形态学分析，以表征其特性，特别强调胞外多糖的生产。菌株GZB16/CEES1，表现出显著的耐药性和产卵氰素。生化和分子分析，包括其16SrRNA基因的测序（登录号LN735036.1），质粒固化试验，和质粒大小的估计，进行了阐明其耐药机制和进一步基于pyocyin的目标，白色念珠菌菌株GZB16/CEES1对研究中使用的各种抗生素表现出100％的耐药性，用质粒固化试验，表明基于质粒的抗性基因传递。估计GZB16/CEES1中的质粒大小约为24kb。本研究的重点是铜绿假单胞菌的绿脓苷生产，揭示了它与抗虫活性的关联。细菌提取物对白色念珠菌的最低抑菌浓度（MIC）为50μg/ml，具有38.5μg/ml的略低的基于绿脓苷的MIC。扫描电子显微镜显示铜绿假单胞菌菌株和白色念珠菌细胞之间的直接相互作用，导致后者的毁灭。
■这些发现强调了铜绿假单胞菌在理解微生物相互作用和制定防治真菌感染策略方面的潜力。该研究强调了研究细菌-真菌相互作用以及绿脓苷在抗菌活性中的作用的重要性。在这一领域的进一步研究可能导致开发新的治疗方法来对抗多药耐药感染。
