PDF(Pubmed)
Abstract：
OBJECTIVE: The Bengal tiger Panthera tigris tigris, is an emblematic animal for Bangladesh. Despite being the apex predator in the wild, their number is decreasing due to anthropogenic activities such as hunting, urbanization, expansion of agriculture and deforestation. By contrast, captive tigers are flourishing due to practical conservation efforts. Breeding within the small captive population can produce inbreeding depression and genetic bottlenecks, which may limit the success of conservation efforts. Despite past decades of research, a comprehensive database on genetic variation in the captive and wild Bengal tigers in Bangladesh still needs to be included. Therefore, this research aimed to investigate the White Bengal tiger genome to create a resource for future studies to understand variation underlying important functional traits.
METHODS: Blood samples from Chattogram Zoo were collected for three white Bengal tigers. Genomic DNA for all collected samples were extracted using a commercial DNA extraction kit. Whole genome sequencing was performed using a DNBseq platform. We generated 77 Gb of whole-genome sequencing (WGS) data for three white Bengal tigers (Average 11X coverage/sample). The data we generated will establish a paradigm for tiger research in Bangladesh by providing a genomic resource for future functional studies on the Bengal white tiger.
METHODS: Blood samples from Chattogram Zoo were collected for three white Bengal tigers. Genomic DNA for all collected samples were extracted using a commercial DNA extraction kit. Whole genome sequencing was performed using a DNBseq platform. We generated 77 Gb of whole-genome sequencing (WGS) data for three white Bengal tigers (Average 11X coverage/sample). The data we generated will establish a paradigm for tiger research in Bangladesh by providing a genomic resource for future functional studies on the Bengal white tiger.
摘要：
目标：孟加拉虎黑豹，是孟加拉国的象征动物。尽管是野外的顶级捕食者，由于狩猎等人为活动，它们的数量正在减少，城市化,扩大农业和森林砍伐。相比之下,由于实际的保护工作，圈养的老虎正在蓬勃发展。小圈养种群内的育种会产生近交抑郁和遗传瓶颈，这可能会限制保护工作的成功。尽管过去几十年的研究，孟加拉国圈养和野生孟加拉虎遗传变异的综合数据库仍需纳入。因此,这项研究旨在调查白孟加拉虎基因组，为未来的研究创造资源，以了解重要功能性状的变化。
方法：从Chattogram动物园收集三只白孟加拉虎的血液样本。使用商业DNA提取试剂盒提取所有收集的样品的基因组DNA。使用DNBseq平台进行全基因组测序。我们为三只白孟加拉虎生成了77Gb的全基因组测序(WGS)数据(平均11X覆盖率/样品)。我们生成的数据将为孟加拉白虎的未来功能研究提供基因组资源，从而为孟加拉国的老虎研究建立范例。
方法：从Chattogram动物园收集三只白孟加拉虎的血液样本。使用商业DNA提取试剂盒提取所有收集的样品的基因组DNA。使用DNBseq平台进行全基因组测序。我们为三只白孟加拉虎生成了77Gb的全基因组测序(WGS)数据(平均11X覆盖率/样品)。我们生成的数据将为孟加拉白虎的未来功能研究提供基因组资源，从而为孟加拉国的老虎研究建立范例。
