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Abstract：
BACKGROUND: Early and appropriate antibiotic treatment improves the clinical outcome of patients with sepsis. There is an urgent need for rapid identification (ID) and antimicrobial susceptibility testing (AST) of bacteria that cause bloodstream infection (BSI). Rapid ID and AST can be achieved by short-term incubation on solid medium of positive blood cultures using MALDI-TOF mass spectrometry (MS) and the BD M50 system. The purpose of this study is to evaluate the performance of rapid method compared to traditional method.
METHODS: A total of 124 mono-microbial samples were collected. Positive blood culture samples were short-term incubated on blood agar plates and chocolate agar plates for 5 ∼ 7 h, and the rapid ID and AST were achieved through Zybio EXS2000 MS and BD M50 System, respectively.
RESULTS: Compared with the traditional 24 h culture for ID, this rapid method can shorten the cultivation time to 5 ∼ 7 h. Accurate organism ID was achieved in 90.6% of Gram-positive bacteria (GP), 98.5% of Gram-negative bacteria (GN), and 100% of fungi. The AST resulted in the 98.5% essential agreement (EA) and 97.1% category agreements (CA) in NMIC-413, 99.4% EA and 98.9% CA in PMIC-92, 100% both EA and CA in SMIC-2. Besides, this method can be used for 67.2% (264/393) of culture bottles during routine work. The mean turn-around time (TAT) for obtaining final results by conventional method is approximately 72.6 ± 10.5 h, which is nearly 24 h longer than the rapid method.
CONCLUSIONS: The newly described method is expected to provide faster and reliable ID and AST results, making it an important tool for rapid management of blood cultures (BCs). In addition, this rapid method can be used to process most positive blood cultures, enabling patients to receive rapid and effective treatment.
METHODS: A total of 124 mono-microbial samples were collected. Positive blood culture samples were short-term incubated on blood agar plates and chocolate agar plates for 5 ∼ 7 h, and the rapid ID and AST were achieved through Zybio EXS2000 MS and BD M50 System, respectively.
RESULTS: Compared with the traditional 24 h culture for ID, this rapid method can shorten the cultivation time to 5 ∼ 7 h. Accurate organism ID was achieved in 90.6% of Gram-positive bacteria (GP), 98.5% of Gram-negative bacteria (GN), and 100% of fungi. The AST resulted in the 98.5% essential agreement (EA) and 97.1% category agreements (CA) in NMIC-413, 99.4% EA and 98.9% CA in PMIC-92, 100% both EA and CA in SMIC-2. Besides, this method can be used for 67.2% (264/393) of culture bottles during routine work. The mean turn-around time (TAT) for obtaining final results by conventional method is approximately 72.6 ± 10.5 h, which is nearly 24 h longer than the rapid method.
CONCLUSIONS: The newly described method is expected to provide faster and reliable ID and AST results, making it an important tool for rapid management of blood cultures (BCs). In addition, this rapid method can be used to process most positive blood cultures, enabling patients to receive rapid and effective treatment.
摘要：
背景：早期和适当的抗生素治疗可改善脓毒症患者的临床预后。迫切需要对引起血流感染(BSI)的细菌进行快速鉴定(ID)和抗微生物药敏试验(AST)。快速ID和AST可以通过使用MALDI-TOF质谱（MS）和BDM50系统在阳性血液培养物的固体培养基上短期孵育来实现。这项研究的目的是评估快速方法与传统方法相比的性能。
方法:收集总共124个单微生物样品。阳性血培养样品在血琼脂平板和巧克力琼脂平板上短期孵育5~7小时，通过ZybioEXS2000MS和BDM50系统实现了快速ID和AST，分别。
结果：与传统的ID培养24小时相比，这种快速方法可以将培养时间缩短至5~7小时。90.6%的革兰氏阳性菌(GP)达到了准确的生物体ID，98.5％的革兰氏阴性菌（GN），和100%的真菌。AST在NMIC-413中产生了98.5％的基本协议（EA）和97.1％的类别协议（CA），在PMIC-92中产生了99.4％的EA和98.9％的CA，在SMIC-2中产生了100％的EA和CA。此外,该方法可用于67.2%(264/393)的培养瓶在日常工作中。通过常规方法获得最终结果的平均周转时间（TAT）约为72.6±10.5h，这比快速方法长了近24小时。
结论：新描述的方法有望提供更快，可靠的ID和AST结果，使其成为快速管理血液培养物（BCs）的重要工具。此外,这种快速方法可用于处理大多数阳性血液培养物，使患者能够得到快速有效的治疗。
方法:收集总共124个单微生物样品。阳性血培养样品在血琼脂平板和巧克力琼脂平板上短期孵育5~7小时，通过ZybioEXS2000MS和BDM50系统实现了快速ID和AST，分别。
结果：与传统的ID培养24小时相比，这种快速方法可以将培养时间缩短至5~7小时。90.6%的革兰氏阳性菌(GP)达到了准确的生物体ID，98.5％的革兰氏阴性菌（GN），和100%的真菌。AST在NMIC-413中产生了98.5％的基本协议（EA）和97.1％的类别协议（CA），在PMIC-92中产生了99.4％的EA和98.9％的CA，在SMIC-2中产生了100％的EA和CA。此外,该方法可用于67.2%(264/393)的培养瓶在日常工作中。通过常规方法获得最终结果的平均周转时间（TAT）约为72.6±10.5h，这比快速方法长了近24小时。
结论：新描述的方法有望提供更快，可靠的ID和AST结果，使其成为快速管理血液培养物（BCs）的重要工具。此外,这种快速方法可用于处理大多数阳性血液培养物，使患者能够得到快速有效的治疗。
