PDF(Pubmed)
Abstract：
Hu sheep are a unique breed in our country with great reproductive potential, the extent of whose breeding has been steadily rising in recent years. The study subjects in this experiment were 8-month-old Hu sheep (n = 112). First of all, the growth performance, slaughter performance and meat quality of their eye muscle quality were assessed, meanwhile their live weight, carcass weight, body length, body height, chest circumference, chest depth and tube circumference were respectively 33.81 ± 5.47 kg, 17.43 ± 3.21 kg, 60.36 ± 4.41 cm, 63.25 ± 3.88 cm, 72.03 ± 5.02 cm, 30.70 ± 2.32 cm and 7.36 ± 0.56 cm, with a significant difference between rams and ewes (P < 0.01). Following that, transcriptome sequencing was done, and candidate genes related to growth performance were identified using the weighted co-expression network analysis (WGCNA) approach, which was used to identified 15 modules, with the turquoise and blue modules having the strongest association with growth and slaughter performance, respectively. We discovered hub genes such as ARHGAP31, EPS8, AKT3, EPN1, PACS2, KIF1C, C12H1orf115, FSTL1, PTGFRN and IFIH1 in the gene modules connected with growth and slaughter performance. Our research identifies the hub genes associated with the growth and slaughter performance of Hu sheep, which play an important role in their muscle growth, organ and cartilage development, blood vessel development and energy metabolic pathways. Our findings might lead to the development of potentially-useful biomarkers for the selection of growth and slaughterer performance-related attributes of sheep and other livestock.
摘要：
湖羊是我国特有的繁殖潜力巨大的品种,近年来，其繁殖程度一直在稳步上升。本实验研究对象为8月龄湖羊(n=112)。首先,增长业绩，对其眼部肌肉质量的屠宰性能和肉质进行了评估，同时他们的活重，屠体重量,身体长度,身体高度,胸围,胸深和管围分别为33.81±5.47kg，17.43±3.21kg,60.36±4.41厘米，63.25±3.88cm,72.03±5.02cm,30.70±2.32厘米和7.36±0.56厘米，公羊和母羊之间差异显著(P<0.01)。在此之后,转录组测序已经完成，并使用加权共表达网络分析(WGCNA)方法鉴定与生长性能相关的候选基因，用于识别15个模块，绿松石和蓝色模块与生长和屠宰性能的关联性最强，分别。我们发现了hub基因，如ARHGAP31，EPS8，AKT3，EPN1，PACS2，KIF1C，C12H1orf115、FSTL1、PTGFRN和IFIH1在基因模块中的衔接与生长和屠宰表现有关。我们的研究确定了与湖羊生长和屠宰性能相关的枢纽基因，在他们的肌肉生长中起着重要作用，器官和软骨发育，血管发育和能量代谢途径。我们的发现可能导致开发潜在有用的生物标志物，用于选择绵羊和其他牲畜的生长和屠宰性能相关属性。
