Abstract：
We investigated suitable culture conditions for the production of the blue pigment phycocyanin (PC) from the unique filamentous cyanobacteria Pseudanabaena sp. ABRG5-3 and Limnothrix sp. SK1-2-1. White, green, or red LED irradiation at 30 μmol photons/m2/s was effective for phycocyanin production when compared with Arthrospira platensis (Spirulina) sp. NIES-39, which is generally grown under high light irradiation. To investigate the safety of the cyanobacteria, ABRG5-3 cells were subjected to Ames (reverse mutation) tests and single oral-dose rat studies, which revealed non-mutagenic and non-toxic properties. When three purified phycocyanins (abPC, skPC, and spPC) were subjected to agarose gel electrophoresis, they showed different mobility, indicating that each phycocyanin has unique properties. abPC exhibited strong antiglycation activities as novel function.
摘要：
我们研究了从独特的丝状蓝细菌Pseudanabaenasp生产蓝色色素藻蓝蛋白（PC）的合适培养条件。ABRG5-3和Limnothrixsp。SK1-2-1.白色,绿色,与螺旋藻（螺旋藻）sp相比，以30μmol光子/m2/s的强度或红色LED照射对藻蓝蛋白的生产有效。NIES-39，通常在强光照射下生长。为了研究蓝藻的安全性，ABRG5-3细胞进行Ames（反向突变）测试和单次口服剂量大鼠研究，揭示了非诱变和无毒特性。当三个纯化的藻蓝蛋白(abPC，skPC,和spPC）进行琼脂糖凝胶电泳，他们表现出不同的流动性，表明每个藻蓝蛋白具有独特的性质。abPC表现出强的抗糖基化活性作为新功能。
