关键词: Monascus ruber Morphology Secondary metabolites Selenite metabolism Transcriptome

Mesh : Monascus / metabolism genetics growth & development Selenium / metabolism Biofortification Selenious Acid / metabolism Citrinin / metabolism Fungal Proteins / genetics metabolism Pigments, Biological / metabolism Fermentation Biological Products

来  源:   DOI:10.1016/j.foodchem.2024.139740

Abstract:
Monascus species are functional fermentation fungi with great potential for selenium (Se) supplementation. This study investigated the effects of Se bio-fortification on the growth, morphology, and biosynthesis of Monascus ruber M7. The results demonstrated a significant increase in the yield of orange and red Monascus pigments (MPs) in red yeast rice (RYR) by 38.52% and 36.57%, respectively, under 20 μg/mL of selenite pressure. Meanwhile, the production of citrinin (CIT), a mycotoxin, decreased from 244.47 μg/g to 175.01 μg/g. Transcriptome analysis revealed significant upregulation of twelve genes involved in MPs biosynthesis, specifically MpigE, MpigF, and MpigN, and downregulation of four genes (mrr3, mrr4, mrr7, and mrr8) associated with CIT biosynthesis. Additionally, three genes encoding cysteine synthase cysK (Log2FC = 1.6), methionine synthase metH (Log2FC = 2.2), and methionyl-tRNA synthetase metG (Log2FC = 1.8) in selenocompound metabolism showed significantly upregulated. These findings provide insights into Se biotransformation and metabolism in filamentous fungi.
摘要:
红曲霉是功能性发酵真菌,具有补充硒(Se)的潜力。本研究调查了硒生物强化对生长的影响,形态学,以及红曲霉M7的生物合成。结果表明,红曲米(RYR)中橙色和红色红曲色素(MPs)的产量显着提高了38.52%和36.57%,分别,在20μg/mL的亚硒酸盐压力下。同时,citrinin(CIT)的生产,一种霉菌毒素,从244.47μg/g降至175.01μg/g。转录组分析显示,参与MPs生物合成的12个基因显著上调,特别是Mpige,MpigF,还有MpigN,以及与CIT生物合成相关的四个基因(mrr3,mrr4,mrr7和mrr8)的下调。此外,三个编码半胱氨酸合酶cysK的基因(Log2FC=1.6),蛋氨酸合成酶metH(Log2FC=2.2),在硒化合物代谢中,甲硫氨酰-tRNA合成酶metG(Log2FC=1.8)显着上调。这些发现为丝状真菌中硒的生物转化和代谢提供了见解。
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