Abstract：
OBJECTIVE: Assessment of WNT1, WNT3a, and LRP6 concentrations in patients with ischemic heart disease (IHD) and obstructive and non-obstructive coronary artery (CA) disease.
METHODS: This cross-sectional observational study included 50 IHD patients (verified by coronary angiography, CAG), of which 25 (50%) were men, mean age 64.9±8.1 years; 20 patients had non-obstructive CA disease (stenosis <50%), and 30 patients had hemodynamically significant stenosis. Concentrations of WNT1, WNT3a and LRP6 were measured in all patients.
RESULTS: The concentrations of WNT1 and WNT3a proteins were significantly higher in patients with IHD and obstructive CA disease (p < 0.001), while the concentration of LRP6 was higher in the group with non-obstructive CA disease (p = 0.016). Data analysis of the group with obstructive CA disease showed a moderate correlation between WNT1 and LRP6 (ρ=0.374; p=0.042). Correlation analysis of all groups of patients with CA disease revealed a moderate association between the concentrations of WNT1 and uric acid (ρ=0.416; p=0.007). Regression analysis showed that risk factors for the development of IHD, such as increased body mass index, age, smoking, dyslipidemia, and hypertension, did not significantly influence the type of CA disease in IHD patients. According to ROC analysis, the obstructive form of IHD was predicted by a WNT3a concentration higher than 0.155 ng/ml and a LRP6 concentration lower than 12.94 ng/ml.
CONCLUSIONS: IHD patients with non-obstructive CA disease had the greatest increase in LRP6, while patients with obstructive CA disease had significantly higher concentrations of the canonical WNT cascade proteins, WNT1 and WNT3a. According to the ROC analysis, a WNT3a concentration >0.155 ng/ml can serve as a predictor for the presence of hemodynamically significant CA stenosis in IHD patients (sensitivity 96.7%; specificity 70%), whereas a LRP6 concentration >12.94 ng/ml can predict the development of non-obstructive CA disease (sensitivity 76.7%; specificity 65%).
METHODS: This cross-sectional observational study included 50 IHD patients (verified by coronary angiography, CAG), of which 25 (50%) were men, mean age 64.9±8.1 years; 20 patients had non-obstructive CA disease (stenosis <50%), and 30 patients had hemodynamically significant stenosis. Concentrations of WNT1, WNT3a and LRP6 were measured in all patients.
RESULTS: The concentrations of WNT1 and WNT3a proteins were significantly higher in patients with IHD and obstructive CA disease (p < 0.001), while the concentration of LRP6 was higher in the group with non-obstructive CA disease (p = 0.016). Data analysis of the group with obstructive CA disease showed a moderate correlation between WNT1 and LRP6 (ρ=0.374; p=0.042). Correlation analysis of all groups of patients with CA disease revealed a moderate association between the concentrations of WNT1 and uric acid (ρ=0.416; p=0.007). Regression analysis showed that risk factors for the development of IHD, such as increased body mass index, age, smoking, dyslipidemia, and hypertension, did not significantly influence the type of CA disease in IHD patients. According to ROC analysis, the obstructive form of IHD was predicted by a WNT3a concentration higher than 0.155 ng/ml and a LRP6 concentration lower than 12.94 ng/ml.
CONCLUSIONS: IHD patients with non-obstructive CA disease had the greatest increase in LRP6, while patients with obstructive CA disease had significantly higher concentrations of the canonical WNT cascade proteins, WNT1 and WNT3a. According to the ROC analysis, a WNT3a concentration >0.155 ng/ml can serve as a predictor for the presence of hemodynamically significant CA stenosis in IHD patients (sensitivity 96.7%; specificity 70%), whereas a LRP6 concentration >12.94 ng/ml can predict the development of non-obstructive CA disease (sensitivity 76.7%; specificity 65%).
摘要：
目的：WNT1、WNT3a、缺血性心脏病（IHD）和阻塞性和非阻塞性冠状动脉（CA）患者的LRP6浓度。
方法：这项横断面观察性研究包括50名IHD患者（经冠状动脉造影证实，CAG),其中25人（50%）是男性，平均年龄64.9±8.1岁；20例患者患有非阻塞性CA疾病（狭窄50%），30例患者有血流动力学显著狭窄。在所有患者中测量WNT1、WNT3a和LRP6的浓度。
结果：患有IHD和阻塞性CA疾病的患者中WNT1和WNT3a蛋白的浓度显着升高（p＆lt;0.001），而非阻塞性CA疾病组的LRP6浓度较高（p=0.016）。阻塞性CA疾病组的数据分析显示WNT1和LRP6之间存在中度相关性（ρ=0.374；p=0.042）。对所有CA疾病患者组的相关性分析显示，WNT1浓度与尿酸之间存在中度关联（ρ=0.416；p=0.007）。回归分析显示,IHD发病的危险因素,例如增加的身体质量指数，年龄,吸烟,血脂异常,和高血压，对IHD患者的CA疾病类型没有显著影响。根据ROC分析,通过WNT3a浓度高于0.155ng/ml和LRP6浓度低于12.94ng/ml来预测IHD的阻塞性形式.
结论：患有非阻塞性CA疾病的IHD患者的LRP6增加最大，而患有阻塞性CA疾病的患者的典型WNT级联蛋白浓度明显更高，WNT1和WNT3a。根据ROC分析,aWNT3a浓度>0.155ng/ml可以作为IHD患者血液动力学显著CA狭窄存在的预测因子(敏感性96.7%;特异性70%),而LRP6浓度>12.94ng/ml可以预测非阻塞性CA疾病的发展(敏感性76.7%;特异性65%)。
方法：这项横断面观察性研究包括50名IHD患者（经冠状动脉造影证实，CAG),其中25人（50%）是男性，平均年龄64.9±8.1岁；20例患者患有非阻塞性CA疾病（狭窄50%），30例患者有血流动力学显著狭窄。在所有患者中测量WNT1、WNT3a和LRP6的浓度。
结果：患有IHD和阻塞性CA疾病的患者中WNT1和WNT3a蛋白的浓度显着升高（p＆lt;0.001），而非阻塞性CA疾病组的LRP6浓度较高（p=0.016）。阻塞性CA疾病组的数据分析显示WNT1和LRP6之间存在中度相关性（ρ=0.374；p=0.042）。对所有CA疾病患者组的相关性分析显示，WNT1浓度与尿酸之间存在中度关联（ρ=0.416；p=0.007）。回归分析显示,IHD发病的危险因素,例如增加的身体质量指数，年龄,吸烟,血脂异常,和高血压，对IHD患者的CA疾病类型没有显著影响。根据ROC分析,通过WNT3a浓度高于0.155ng/ml和LRP6浓度低于12.94ng/ml来预测IHD的阻塞性形式.
结论：患有非阻塞性CA疾病的IHD患者的LRP6增加最大，而患有阻塞性CA疾病的患者的典型WNT级联蛋白浓度明显更高，WNT1和WNT3a。根据ROC分析,aWNT3a浓度>0.155ng/ml可以作为IHD患者血液动力学显著CA狭窄存在的预测因子(敏感性96.7%;特异性70%),而LRP6浓度>12.94ng/ml可以预测非阻塞性CA疾病的发展(敏感性76.7%;特异性65%)。
