PDF(Pubmed)
Abstract：
UNASSIGNED: This study aimed to investigate the feasibility and effectiveness of using indocyanine green (ICG) injected intracutaneously through the lower limbs and perineum for visualized tracking, localization, and qualitative assessment of pelvic lymph nodes (LNs) in bladder cancer to achieve their accurate resection.
UNASSIGNED: First, ICG was injected into the LN metastasis model mice lower limbs, and real-time and dynamic in vivo and ex vivo imaging was conducted by using a near-infrared fluorescence imaging system. Additionally, 26 patients with bladder cancer were enrolled and divided into intracutaneous group and transurethral group. A near-infrared fluorescence imaging device with internal and external imaging probes was used to perform real-time tracking, localization, and resection of the pelvic LNs.
UNASSIGNED: The mice normal LNs and the metastatic LNs exhibited fluorescence. The metastatic LNs showed a significantly higher signal-to-background ratio than the normal LNs (3.9 ± 0.2 vs. 2.0 ± 0.1, p < 0.05). In the intracutaneous group, the accuracy rate of fluorescent-labeled LNs was 97.6%, with an average of 11.3 ± 2.4 LNs resected per patient. Six positive LNs were detected in three patients (18.8%). In the transurethral group, the accuracy rate of fluorescent-labeled LNs was 84.4%, with an average of 8.6 ± 2.3 LNs resected per patient. Two positive LNs were detected in one patient (12.5%).
UNASSIGNED: Following the intracutaneous injection of ICG into the lower limbs and perineum, the dye accumulates in pelvic LNs through lymphatic reflux. By using near-infrared fluorescence laparoscopic fusion imaging, physicians can perform real-time tracking, localization, and precise resection of pelvic LNs.
UNASSIGNED: First, ICG was injected into the LN metastasis model mice lower limbs, and real-time and dynamic in vivo and ex vivo imaging was conducted by using a near-infrared fluorescence imaging system. Additionally, 26 patients with bladder cancer were enrolled and divided into intracutaneous group and transurethral group. A near-infrared fluorescence imaging device with internal and external imaging probes was used to perform real-time tracking, localization, and resection of the pelvic LNs.
UNASSIGNED: The mice normal LNs and the metastatic LNs exhibited fluorescence. The metastatic LNs showed a significantly higher signal-to-background ratio than the normal LNs (3.9 ± 0.2 vs. 2.0 ± 0.1, p < 0.05). In the intracutaneous group, the accuracy rate of fluorescent-labeled LNs was 97.6%, with an average of 11.3 ± 2.4 LNs resected per patient. Six positive LNs were detected in three patients (18.8%). In the transurethral group, the accuracy rate of fluorescent-labeled LNs was 84.4%, with an average of 8.6 ± 2.3 LNs resected per patient. Two positive LNs were detected in one patient (12.5%).
UNASSIGNED: Following the intracutaneous injection of ICG into the lower limbs and perineum, the dye accumulates in pelvic LNs through lymphatic reflux. By using near-infrared fluorescence laparoscopic fusion imaging, physicians can perform real-time tracking, localization, and precise resection of pelvic LNs.
摘要：
■本研究旨在探讨通过下肢和会阴皮下注射吲哚菁绿（ICG）进行可视化跟踪的可行性和有效性，本地化,并对膀胱癌盆腔淋巴结（LNs）进行定性评估，以实现其准确切除。
■首先，将ICG注射到LN转移模型小鼠下肢,使用近红外荧光成像系统进行实时动态的体内和离体成像。此外,纳入26例膀胱癌患者，分为皮内组和经尿道组。使用具有内部和外部成像探针的近红外荧光成像设备进行实时跟踪，本地化,和切除骨盆LN。
■小鼠正常LN和转移性LN表现出荧光。转移性LN显示出明显高于正常LN的信号背景比（3.9±0.2vs.2.0±0.1，p<0.05)。在皮内组中，荧光标记LN的准确率为97.6%,每位患者平均切除11.3±2.4LN。在3例患者中检测到6个阳性LN（18.8%）。在经尿道组,荧光标记LN的准确率为84.4%，每位患者平均切除8.6±2.3LN。在一名患者中检测到两个阳性LN（12.5％）。
■将ICG皮内注射到下肢和会阴后，染料通过淋巴回流积聚在骨盆LN中。利用近红外荧光技术进行腹腔镜融合成像,医生可以进行实时跟踪，本地化,和骨盆LN的精确切除。
■首先，将ICG注射到LN转移模型小鼠下肢,使用近红外荧光成像系统进行实时动态的体内和离体成像。此外,纳入26例膀胱癌患者，分为皮内组和经尿道组。使用具有内部和外部成像探针的近红外荧光成像设备进行实时跟踪，本地化,和切除骨盆LN。
■小鼠正常LN和转移性LN表现出荧光。转移性LN显示出明显高于正常LN的信号背景比（3.9±0.2vs.2.0±0.1，p<0.05)。在皮内组中，荧光标记LN的准确率为97.6%,每位患者平均切除11.3±2.4LN。在3例患者中检测到6个阳性LN（18.8%）。在经尿道组,荧光标记LN的准确率为84.4%，每位患者平均切除8.6±2.3LN。在一名患者中检测到两个阳性LN（12.5％）。
■将ICG皮内注射到下肢和会阴后，染料通过淋巴回流积聚在骨盆LN中。利用近红外荧光技术进行腹腔镜融合成像,医生可以进行实时跟踪，本地化,和骨盆LN的精确切除。
