UNASSIGNED: First, ICG was injected into the LN metastasis model mice lower limbs, and real-time and dynamic in vivo and ex vivo imaging was conducted by using a near-infrared fluorescence imaging system. Additionally, 26 patients with bladder cancer were enrolled and divided into intracutaneous group and transurethral group. A near-infrared fluorescence imaging device with internal and external imaging probes was used to perform real-time tracking, localization, and resection of the pelvic LNs.
UNASSIGNED: The mice normal LNs and the metastatic LNs exhibited fluorescence. The metastatic LNs showed a significantly higher signal-to-background ratio than the normal LNs (3.9 ± 0.2 vs. 2.0 ± 0.1, p < 0.05). In the intracutaneous group, the accuracy rate of fluorescent-labeled LNs was 97.6%, with an average of 11.3 ± 2.4 LNs resected per patient. Six positive LNs were detected in three patients (18.8%). In the transurethral group, the accuracy rate of fluorescent-labeled LNs was 84.4%, with an average of 8.6 ± 2.3 LNs resected per patient. Two positive LNs were detected in one patient (12.5%).
UNASSIGNED: Following the intracutaneous injection of ICG into the lower limbs and perineum, the dye accumulates in pelvic LNs through lymphatic reflux. By using near-infrared fluorescence laparoscopic fusion imaging, physicians can perform real-time tracking, localization, and precise resection of pelvic LNs.
■首先,将ICG注射到LN转移模型小鼠下肢,使用近红外荧光成像系统进行实时动态的体内和离体成像。此外,纳入26例膀胱癌患者,分为皮内组和经尿道组。使用具有内部和外部成像探针的近红外荧光成像设备进行实时跟踪,本地化,和切除骨盆LN。
■小鼠正常LN和转移性LN表现出荧光。转移性LN显示出明显高于正常LN的信号背景比(3.9±0.2vs.2.0±0.1,p<0.05)。在皮内组中,荧光标记LN的准确率为97.6%,每位患者平均切除11.3±2.4LN。在3例患者中检测到6个阳性LN(18.8%)。在经尿道组,荧光标记LN的准确率为84.4%,每位患者平均切除8.6±2.3LN。在一名患者中检测到两个阳性LN(12.5%)。
■将ICG皮内注射到下肢和会阴后,染料通过淋巴回流积聚在骨盆LN中。利用近红外荧光技术进行腹腔镜融合成像,医生可以进行实时跟踪,本地化,和骨盆LN的精确切除。