Abstract：
<b>Background and Objective:</b> Gamma irradiation induces genotoxicity, characterized by the formation of extra-nuclear bodies and left behind during the anaphase stage of cell division, often referred to as a micronucleus (MN). The present work aims to monitor exposure to ionizing radiation as a genotoxic agent in the lymphocytes of workers at radiation energy centers. <b>Materials and Methods:</b> The lymphocyte cytokinesis block micronucleus assay used and analyzed the correlation between the Nuclear Division Index (NDI), age, blood type and the number of micronuclei (MN). Blood samples were collected from 20 volunteers in heparin tubes, exposed to 2 Gy gamma rays and cultured <i>in vitro</i>. <b>Results:</b> A significant difference in the number of micronuclei between blood group A and blood groups A, B and AB. The Nuclear Division Index (NDI) value for lymphocytes of radiation energy center workers after gamma radiation was significant (1.74±0.1) but still within the normal range. Neither MN frequency nor NDI values correlated with age, but MN frequency showed a correlation with blood type. <b>Conclusion:</b> The gamma irradiation did not induce a cytostatic effect but proved genotoxic to the lymphocytes of radiation energy center workers. Notably, blood type A demonstrated higher sensitivity to gamma radiation.
摘要：
<b>背景和目的:</b>γ射线辐射诱导遗传毒性,以核外体的形成为特征，并在细胞分裂的后期阶段留下，通常被称为微核（MN）。本工作旨在监测辐射能量中心工人淋巴细胞中作为遗传毒性剂的电离辐射暴露。<b>材料与方法:</b>采用淋巴细胞胞质分裂阻滞微核试验,分析核分裂指数(NDI)的相关性,年龄,血型和微核（MN）的数量。从肝素管中收集20名志愿者的血液样本，暴露于2Gy伽马射线，体外培养<i>/i>。<b>结果:</b>A血型与A血型的微核数有显著差异,B和AB。γ辐射后，辐射能源中心工作人员淋巴细胞的核分裂指数（NDI）值很显着（1.74±0.1），但仍在正常范围内。MN频率和NDI值与年龄无关，但是MN频率与血型有关。<b>结论:</b>γ辐射没有诱导细胞抑制作用，但证明对辐射能量中心工作人员的淋巴细胞具有遗传毒性。值得注意的是,A型血对伽马射线的敏感性更高。
