PDF(Pubmed)
Abstract：
BACKGROUND: Peanut (Arachis hypogaea), a vital oil and food crop globally, is susceptible to web blotch which is a significant foliar disease caused by Phoma arachidicola Marasas Pauer&Boerema leading to substantial yield losses in peanut production. Calcium treatment has been found to enhance plant resistance against pathogens.
RESULTS: This study investigates the impact of exogenous calcium on peanut resistance to web blotch and explores its mechanisms. Greenhouse experiments revealed that exogenous calcium treatment effectively enhanced resistance to peanut web blotch. Specifically, amino acid calcium and sugar alcohol calcium solutions demonstrated the best induced resistance effects, achieving reduction rates of 61.54% and 60% in Baisha1016, and 53.94% and 50% in Luhua11, respectively. All exogenous calcium treatments reduced malondialdehyde (MDA) and relative electrical conductivity (REC) levels in peanut leaves, mitigating pathogen-induced cell membrane damage. Exogenous calcium supplementation led to elevated hydrogen peroxide (H2O2) content and superoxide anion (O2∙-) production in peanut leaves, facilitating the accumulation of reactive oxygen species (ROS) crucial for plant defense responses. Amino acid calcium and sugar alcohol calcium treatments significantly boosted activities of peroxidase (POD), superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) in peanut leaves. Activation of these antioxidant enzymes effectively scavenged excess ROS, maintaining ROS balance and mitigating cellular damage.
CONCLUSIONS: In summary, exogenous calcium treatment triggered ROS production, which was subsequently eliminated by the activation of antioxidant enzymes, thereby reducing cell membrane damage and inducing defense responses against peanut web blotch.
RESULTS: This study investigates the impact of exogenous calcium on peanut resistance to web blotch and explores its mechanisms. Greenhouse experiments revealed that exogenous calcium treatment effectively enhanced resistance to peanut web blotch. Specifically, amino acid calcium and sugar alcohol calcium solutions demonstrated the best induced resistance effects, achieving reduction rates of 61.54% and 60% in Baisha1016, and 53.94% and 50% in Luhua11, respectively. All exogenous calcium treatments reduced malondialdehyde (MDA) and relative electrical conductivity (REC) levels in peanut leaves, mitigating pathogen-induced cell membrane damage. Exogenous calcium supplementation led to elevated hydrogen peroxide (H2O2) content and superoxide anion (O2∙-) production in peanut leaves, facilitating the accumulation of reactive oxygen species (ROS) crucial for plant defense responses. Amino acid calcium and sugar alcohol calcium treatments significantly boosted activities of peroxidase (POD), superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) in peanut leaves. Activation of these antioxidant enzymes effectively scavenged excess ROS, maintaining ROS balance and mitigating cellular damage.
CONCLUSIONS: In summary, exogenous calcium treatment triggered ROS production, which was subsequently eliminated by the activation of antioxidant enzymes, thereby reducing cell membrane damage and inducing defense responses against peanut web blotch.
摘要：
背景：花生（花生），全球重要的石油和粮食作物，容易受到网状斑点的影响，这是一种由PhomaarachindicolaMarasasPauer和Boerema引起的严重的叶面疾病，导致花生生产中的大量产量损失。已发现钙处理可增强植物对病原体的抗性。
结果：本研究调查了外源钙对花生抗网斑的影响，并探讨了其机制。温室实验表明，外源钙处理有效增强了对花生网斑点的抗性。具体来说,氨基酸钙和糖醇钙溶液表现出最佳的诱导抗性效果，Baisha1016的减排率分别为61.54%和60%，鲁华11的减排率分别为53.94%和50%。所有外源钙处理均降低了花生叶片中的丙二醛（MDA）和相对电导率（REC）水平，减轻病原体诱导的细胞膜损伤。外源补充钙导致花生叶片中过氧化氢（H2O2）含量和超氧阴离子（O2*-）的产生升高，促进对植物防御反应至关重要的活性氧（ROS）的积累。氨基酸钙和糖醇钙处理显着提高了过氧化物酶（POD）的活性，超氧化物歧化酶（SOD），过氧化氢酶(CAT),和花生叶中的抗坏血酸过氧化物酶（APX）。这些抗氧化酶的激活有效清除过量的ROS，维持ROS平衡，减轻细胞损伤。
结论：总之，外源钙处理触发了ROS的产生，随后被抗氧化酶的激活所消除，从而减少细胞膜损伤并诱导针对花生网斑点的防御反应。
结果：本研究调查了外源钙对花生抗网斑的影响，并探讨了其机制。温室实验表明，外源钙处理有效增强了对花生网斑点的抗性。具体来说,氨基酸钙和糖醇钙溶液表现出最佳的诱导抗性效果，Baisha1016的减排率分别为61.54%和60%，鲁华11的减排率分别为53.94%和50%。所有外源钙处理均降低了花生叶片中的丙二醛（MDA）和相对电导率（REC）水平，减轻病原体诱导的细胞膜损伤。外源补充钙导致花生叶片中过氧化氢（H2O2）含量和超氧阴离子（O2*-）的产生升高，促进对植物防御反应至关重要的活性氧（ROS）的积累。氨基酸钙和糖醇钙处理显着提高了过氧化物酶（POD）的活性，超氧化物歧化酶（SOD），过氧化氢酶(CAT),和花生叶中的抗坏血酸过氧化物酶（APX）。这些抗氧化酶的激活有效清除过量的ROS，维持ROS平衡，减轻细胞损伤。
结论：总之，外源钙处理触发了ROS的产生，随后被抗氧化酶的激活所消除，从而减少细胞膜损伤并诱导针对花生网斑点的防御反应。
