Abstract：
Diet-derived exosome-like nanovesicles are a class of natural active substances that have similar structures and functions to mammalian exosomes. Biyang floral mushrooms and their active extracts have been found to possess radioprotective effects and to deeply explore their novel active substances, the radioprotective effects of Biyang floral mushroom-derived exosome-like nanovesicles (BFMELNs) were investigated in this study. Results showed that these surface-negatively charged vesicles possessed an ideal size and good stability against environmental changes such as temperature and gastrointestinal digestion. Furthermore, BFMELNs could effectively be taken up by HL-7702 cells and Caco-2 cells through cellular phagocytosis mediated by clathrin and dynein. Emphatically, BFMELNs with an exosome-like morphology contained RNA, proteins, lipids, polyphenols and flavonoids to exert good antioxidant and radioprotective effects in vitro. Meanwhile, BFMELNs also exhibited good radioprotective effects by restoring peripheral blood indexes, mitigating damage to organs, and regulating the redox state in mice. Collectively, BFMELNs showed promise as novel and natural radioprotective nano-agents for preventing IR-induced oxidative stress damage.
摘要：
饮食来源的外泌体样纳米囊泡是一类与哺乳动物外泌体具有相似结构和功能的天然活性物质。泌阳花蘑菇及其活性提取物已被发现具有辐射防护作用，并深入探索其新型活性物质，本研究研究了泌阳花蘑菇来源的外泌体样纳米囊泡（BFMELNs）的辐射防护作用。结果表明，这些带表面负电荷的囊泡具有理想的尺寸，并且对温度和胃肠道消化等环境变化具有良好的稳定性。此外，BFMELN可以通过网格蛋白和动力蛋白介导的细胞吞噬作用被HL-7702细胞和Caco-2细胞有效吸收。强调,具有外泌体样形态的BFMELN含有RNA，蛋白质，脂质，多酚和黄酮在体外发挥良好的抗氧化和辐射防护作用。同时,BFMELNs还通过恢复外周血指标表现出良好的辐射防护作用，减轻对器官的损害，调节小鼠的氧化还原状态。总的来说，BFMELNs有望作为新型和天然的辐射防护纳米剂，用于预防IR诱导的氧化应激损伤。
