Abstract：
While polydopamine (PDA) possesses the surface-independent adhesion property of mussel-binding proteins, significant differences exist between them. Particularly, PDA\'s short and rigid backbone differs from the long and flexible protein sequence of mussel-binding proteins. Given that adhesion relies on achieving a conformal contact with large surface coverage, PDA has drawbacks as an adhesive. In our study, we investigated the roles of each building block of PDA to build a better understanding of their binding mechanisms. Initially, we anticipated that catecholamine oligomers form specific binding with substrates. However, our study showed that the universal adhesion of PDA is initiated by the solubility limit of growing oligomers by forming agglomerates, complemented by multiple binding modes of catechol. Notably, in the absence of amines, poly(catechol) either remained in solution or formed minor suspensions without any surface coating, underscoring the essential role of amines in the adhesion process by facilitating insoluble aggregate formation. To substantiate our findings, we induced poly(catechol) aggregation using quaternized poly(4-vinylpyridine) (qPVP), leading to subsequent surface adhesion upon agglomerate formation.
摘要：
虽然聚多巴胺（PDA）具有贻贝结合蛋白的表面非依赖性粘附特性，它们之间存在显著差异。特别是,PDA的短而刚性的骨架不同于贻贝结合蛋白的长而柔性的蛋白质序列。鉴于粘附依赖于实现与大表面覆盖的共形接触,PDA作为粘合剂具有缺点。在我们的研究中,我们调查了PDA每个构建模块的作用,以便更好地理解它们的绑定机制.最初，我们预计儿茶酚胺低聚物与底物形成特异性结合。然而,我们的研究表明，PDA的普遍粘附是由通过形成附聚物而生长的低聚物的溶解度极限引发的，辅以儿茶酚的多种结合模式。值得注意的是,在没有胺的情况下，聚（儿茶酚）保留在溶液中或形成少量悬浮液，没有任何表面涂层，通过促进不溶性聚集体的形成，强调胺在粘附过程中的重要作用。为了证实我们的发现,我们使用季铵化聚（4-乙烯基吡啶）（qPVP）诱导聚（儿茶酚）聚集，导致随后在结块形成时的表面粘附。
