Abstract：
Volatile thiol 3-mercaptohexan-1-ol (3MH) and particularly 4-mercapto-4-methylpentan-2-one (4MMP) are highly potent flavour compounds in hops. For the determination, a simple and robust stable isotope dilution LC-MS/MS method was developed and applied to 32 hop varieties worldwide from harvest years 2019 and 2020. Limit of detection, precision, and recovery were 0.15 μg/kg, 10%, and 97-108%, respectively. Levels of 3MH and 4MMP ranged from 1.9 to 79.2 μg/kg and from undetectable to 37.1 μg/kg, respectively. Citra, Mosaic, and Strata were rich in both thiols. ICP analyses revealed, that variation of potassium content between the two harvest years was inversely correlated with that of manganese and rubidium (|r| ≥ 0.89) among 12 US varieties excluding Citra and Mosaic. Total essential oil content (0.34-2.7 mL/100 g) was inversely correlated with calcium content (|r| ≥ 0.65). Greatly varying thiol levels depending on variety, region and harvest year might lead to differing flavour results in beer.
摘要：
挥发性硫醇3-巯基己-1-醇（3MH），特别是4-巯基-4-甲基戊-2-酮（4MMP）是啤酒花中的高效风味化合物。为了确定，开发了一种简单而强大的稳定同位素稀释LC-MS/MS方法，并从2019年和2020年开始将其应用于全球32个啤酒花品种。检测限,精度,回收率为0.15μg/kg，10%,97-108%,分别。3MH和4MMP的水平范围为1.9至79.2μg/kg，检测不到至37.1μg/kg，分别。Citra,马赛克,和地层富含两种硫醇。ICP分析显示，在不包括Citra和Mosaic的12个美国品种中，两个收获年之间的钾含量变化与锰和铷的变化呈负相关（|r|≥0.89）。总精油含量（0.34-2.7mL/100g）与钙含量呈负相关（|r|≥0.65）。根据品种不同，硫醇水平变化很大，地区和收获年份可能会导致不同的啤酒口味结果。
