PDF(Pubmed)
Abstract：
UNASSIGNED: The importance of the gut microbiota in maintaining bone homeostasis has been increasingly emphasized by recent research. This study aimed to identify whether and how the gut microbiome of postmenopausal women with osteoporosis and osteopenia may differ from that of healthy individuals.
UNASSIGNED: Fecal samples were collected from 27 individuals with osteoporosis (OP), 44 individuals with osteopenia (ON), and 23 normal controls (NC). The composition of the gut microbial community was analyzed by 16S rRNA gene sequencing.
UNASSIGNED: No significant difference was found in the microbial composition between the three groups according to alpha and beta diversity. At the phylum level, Proteobacteria and Fusobacteriota were significantly higher and Synergistota was significantly lower in the ON group than in the NC group. At the genus level, Roseburia, Clostridia_UCG.014, Agathobacter, Dialister and Lactobacillus differed between the OP and NC groups as well as between the ON and NC groups (p < 0.05). Linear discriminant effect size (LEfSe) analysis results showed that one phylum community and eighteen genus communities were enriched in the NC, ON and OP groups, respectively. Spearman correlation analysis showed that the abundance of the Dialister genus was positively correlated with BMD and T score at the lumbar spine (p < 0.05). Functional predictions revealed that pathways relevant to amino acid biosynthesis, vitamin biosynthesis, and nucleotide metabolism were enriched in the NC group. On the other hand, pathways relevant to metabolites degradation and carbohydrate metabolism were mainly enriched in the ON and OP groups respectively.
UNASSIGNED: Our findings provide new epidemiologic evidence regarding the relationship between the gut microbiota and postmenopausal bone loss, laying a foundation for further exploration of therapeutic targets for the prevention and treatment of postmenopausal osteoporosis (PMO).
UNASSIGNED: Fecal samples were collected from 27 individuals with osteoporosis (OP), 44 individuals with osteopenia (ON), and 23 normal controls (NC). The composition of the gut microbial community was analyzed by 16S rRNA gene sequencing.
UNASSIGNED: No significant difference was found in the microbial composition between the three groups according to alpha and beta diversity. At the phylum level, Proteobacteria and Fusobacteriota were significantly higher and Synergistota was significantly lower in the ON group than in the NC group. At the genus level, Roseburia, Clostridia_UCG.014, Agathobacter, Dialister and Lactobacillus differed between the OP and NC groups as well as between the ON and NC groups (p < 0.05). Linear discriminant effect size (LEfSe) analysis results showed that one phylum community and eighteen genus communities were enriched in the NC, ON and OP groups, respectively. Spearman correlation analysis showed that the abundance of the Dialister genus was positively correlated with BMD and T score at the lumbar spine (p < 0.05). Functional predictions revealed that pathways relevant to amino acid biosynthesis, vitamin biosynthesis, and nucleotide metabolism were enriched in the NC group. On the other hand, pathways relevant to metabolites degradation and carbohydrate metabolism were mainly enriched in the ON and OP groups respectively.
UNASSIGNED: Our findings provide new epidemiologic evidence regarding the relationship between the gut microbiota and postmenopausal bone loss, laying a foundation for further exploration of therapeutic targets for the prevention and treatment of postmenopausal osteoporosis (PMO).
摘要：
最近的研究越来越强调肠道微生物群在维持骨骼稳态中的重要性。这项研究旨在确定患有骨质疏松症和骨质减少的绝经后妇女的肠道微生物组是否以及如何与健康个体不同。
■收集了27名骨质疏松症（OP）患者的粪便样本，44例骨量减少（ON），和23个正常对照（NC）。通过16SrRNA基因测序分析了肠道微生物群落的组成。
■根据α和β多样性，三组之间的微生物组成未发现显着差异。在门一级，与NC组相比，ON组的变形杆菌和Fusobacteriota显着升高，而Synergistota显着降低。在属一级，罗斯布里亚,梭菌_UCG.014，不动杆菌属，Dialister和乳杆菌在OP和NC组之间以及在ON和NC组之间存在差异（p<0.05）。线性判别效应大小（LEfSe）分析结果表明，NC中富集了1个门群落和18个属群落，ON和OP组，分别。Spearman相关分析表明，Dialister属的丰度与腰椎的BMD和T评分呈正相关（p<0.05）。功能预测表明，与氨基酸生物合成相关的途径，维生素生物合成，和核苷酸代谢在NC组中富集。另一方面,与代谢产物降解和碳水化合物代谢相关的通路主要分别在ON和OP组富集。
■我们的研究结果提供了关于肠道菌群与绝经后骨丢失之间关系的新的流行病学证据。为进一步探索防治绝经后骨质疏松症(PMO)的治疗靶点奠定基础。
■收集了27名骨质疏松症（OP）患者的粪便样本，44例骨量减少（ON），和23个正常对照（NC）。通过16SrRNA基因测序分析了肠道微生物群落的组成。
■根据α和β多样性，三组之间的微生物组成未发现显着差异。在门一级，与NC组相比，ON组的变形杆菌和Fusobacteriota显着升高，而Synergistota显着降低。在属一级，罗斯布里亚,梭菌_UCG.014，不动杆菌属，Dialister和乳杆菌在OP和NC组之间以及在ON和NC组之间存在差异（p<0.05）。线性判别效应大小（LEfSe）分析结果表明，NC中富集了1个门群落和18个属群落，ON和OP组，分别。Spearman相关分析表明，Dialister属的丰度与腰椎的BMD和T评分呈正相关（p<0.05）。功能预测表明，与氨基酸生物合成相关的途径，维生素生物合成，和核苷酸代谢在NC组中富集。另一方面,与代谢产物降解和碳水化合物代谢相关的通路主要分别在ON和OP组富集。
■我们的研究结果提供了关于肠道菌群与绝经后骨丢失之间关系的新的流行病学证据。为进一步探索防治绝经后骨质疏松症(PMO)的治疗靶点奠定基础。
