PDF(Pubmed)
Abstract：
Elastin-like polymers are a class of stimuli-responsive protein polymers that hold immense promise in applications such as drug delivery, hydrogels, and biosensors. Yet, understanding the intricate interplay of factors influencing their stimuli-responsive behavior remains a challenging frontier. Using temperature-controlled dynamic light scattering and zeta potential measurements, we investigate the interactions between buffer, pH, salt, water, and protein using an elastin-like polymer containing ionizable lysine residues. We observed the elevation of transition temperature in the presence of the common buffering agent HEPES at low concentrations, suggesting a \"salting-in\" effect of HEPES as a cosolute through weak association with the protein. Our findings motivate a more comprehensive investigation of the influence of buffer and other cosolute molecules on elastin-like polymer behavior.
摘要：
类弹性蛋白聚合物是一类刺激响应性蛋白质聚合物，在药物递送等应用中具有巨大的前景。水凝胶,和生物传感器。然而，了解影响其刺激响应行为的因素的复杂相互作用仍然是一个具有挑战性的前沿。使用温度控制的动态光散射和zeta电位测量，我们研究缓冲区之间的相互作用，pH值,盐,水,和使用含有可电离赖氨酸残基的弹性蛋白样聚合物的蛋白质。我们观察到在低浓度的常见缓冲剂HEPES存在下转变温度的升高，通过与蛋白质的弱结合，表明HEPES作为溶质的“盐析”效应。我们的发现激发了对缓冲液和其他溶质分子对弹性蛋白样聚合物行为的影响的更全面的研究。
