Abstract：
Iron is an essential element in the composition of living organisms and plays a crucial role in a wide range of biological activities. The human body primarily obtains essential iron through the consumption of food. Therefore, it is vital for the health of human body to maintain iron homeostasis. The reducing character of the cellular microenvironment enables Fe2+ to occupy a dominant position within the cell. Hence, there is an urgent need for a simple and sensitive tool that can detect a large amount of Fe2+ in organisms. In this work, a highly specific fluorescent chemodosimeter NPCO (\"NP\" represents the naphthalimide fluorophore, and \"CO\" represents the carbamoyl oxime structure) for the detection of Fe2+ with excellent sensitivity (LOD = 82 nM) was constructed by incorporating a novel carbamoyl oxime structure as the recognition group. NPCO can be effectively employed for the detection of Fe2+ in food samples, living cells, and zebrafish. Furthermore, by using soybean sprouts as a model plant, the application of NPCO was expanded to detect Fe2+ in plants. Therefore, NPCO could be used as an excellent assay tool for detecting Fe2+ in organisms and is expected to be an important aid in exploring the mechanism of iron regulation.
摘要：
铁是生物体组成中的必需元素，在广泛的生物活动中起着至关重要的作用。人体主要通过消耗食物获得必需的铁。因此,维持铁稳态对人体健康至关重要。细胞微环境的还原特性使Fe2+在细胞内占据优势位置。因此，迫切需要一种简单而灵敏的工具，可以检测生物体中的大量Fe2。在这项工作中,一种高度特异性的荧光化学剂量计NPCO(“NP”代表萘酰亚胺荧光团，和“CO”代表氨基甲酰基肟结构），通过掺入新型氨基甲酰基肟结构作为识别基团，以出色的灵敏度（LOD=82nM）检测Fe2。NPCO可有效用于食品样品中Fe2+的检测,活细胞,还有斑马鱼.此外，通过使用豆芽作为模型植物，扩大了NPCO在植物中检测Fe2+的应用范围。因此,NPCO可作为检测生物体中Fe2+的优良检测工具，有望成为探索铁调控机制的重要辅助手段。
