Abstract：
Cryptosporidium spp. are protozoa commonly found in domestic and wild animals. Limited information is available on Cryptosporidium in deer worldwide. In this study, 201 fecal samples were collected from Alpine musk deer on three farms in Gansu Province, China. Detection and subtyping of Cryptosporidium were performed by PCR and sequence analysis of the SSU rRNA and gp60 genes. The prevalence of Cryptosporidium infection in Alpine musk deer was 3.9% (8/201), with infection rates of 1.0% (1/100), 2.8% (1/36), and 9.2% (6/65) in three different farms. All positive samples for Cryptosporidium were from adult deer. Two Cryptosporidium species were identified, including C. parvum (n = 2) and C. xiaoi (n = 6). The C. parvum isolates were subtyped as IIdA15G1, while the C. xiaoi isolates were subtyped as XXIIIa (n = 2) and XXIIIg (n = 4). The IIdA15G1 subtype of C. parvum was found for the first time in deer. These results provide important insights into the identity and human infectious potential of Cryptosporidium in farmed Alpine musk deer.
摘要：
隐孢子虫。是常见于家畜和野生动物中的原生动物。关于全球鹿中隐孢子虫的信息有限。在这项研究中,从甘肃省三个农场的高山麝香鹿中收集了201份粪便样本，中国。通过PCR和SSUrRNA和gp60基因的序列分析进行隐孢子虫的检测和分型。高山麝鹿隐孢子虫感染率为3.9%(8/201),感染率为1.0%(1/100)，2.8%（1/36），三个不同农场的比率为9.2%(6/65)。隐孢子虫的所有阳性样品均来自成年鹿。确定了两种隐孢子虫，包括C.parvum（n=2）和C.xiaoi（n=6）。C.parvum分离株的亚型为IIdA15G1，而C.xiaoi分离株的亚型为XXIIIa（n=2）和XXIIIg（n=4）。在鹿中首次发现了C.parvum的IIdA15G1亚型。这些结果为养殖高山麝香鹿中隐孢子虫的身份和人类感染潜力提供了重要见解。
