Abstract：
UNASSIGNED: Hemolytic disease of the newborn (HDN) is a common condition that can have a severe impact on the health of newborns due to the hemolytic reactions it triggers. Although numerous studies have focused on understanding the pathogenesis of HDN, there are still many unanswered questions.
UNASSIGNED: In this retrospective study, serum samples were collected from 15 healthy newborns and 8 infants diagnosed with hemolytic disease. The relationship between different metabolites and various IgG subtypes in Healthy, HDN and BLI groups was studied by biochemical technique and enzyme-linked immunosorbent assay (ELISA). Metabolomics analysis was conducted to identify the differential metabolites associated with HDN. Subsequently, Pearson\'s correlation analysis was used to determine the relation of these differential metabolites with IgG isoforms. The relationship between the metabolites and IgG subtypes was observed after treatment.
UNASSIGNED: The study results revealed that infants with hemolytic disease exhibited abnormal elevations in TBA, IgG1, IgG2a, IgG2b, IgG3, and IgG4 levels when compared to healthy newborns. Additionally, differences in metabolite contents were also observed. N, N-DIMETHYLARGININE showed negative correlations with TBA, IgG1, IgG2a, IgG2b, IgG3, and IgG4, while 2-HYDROXYBUTYRATE, AMINOISOBUTANOATE, Inosine, and ALLYL ISOTHIOCYANATE exhibited positive correlations with TBA, IgG1, IgG2a, IgG2b, IgG3, and IgG4. Through metabolomics-based research, we have discovered associations between differential metabolites and different IgG isoforms during the onset of HDN.
UNASSIGNED: These findings suggest that changes in metabolite and IgG isoform levels are linked to HDN. Understanding the involvement of IgG isoforms and metabolites can provide valuable guidance for the diagnosis and treatment of HDN.
UNASSIGNED: In this retrospective study, serum samples were collected from 15 healthy newborns and 8 infants diagnosed with hemolytic disease. The relationship between different metabolites and various IgG subtypes in Healthy, HDN and BLI groups was studied by biochemical technique and enzyme-linked immunosorbent assay (ELISA). Metabolomics analysis was conducted to identify the differential metabolites associated with HDN. Subsequently, Pearson\'s correlation analysis was used to determine the relation of these differential metabolites with IgG isoforms. The relationship between the metabolites and IgG subtypes was observed after treatment.
UNASSIGNED: The study results revealed that infants with hemolytic disease exhibited abnormal elevations in TBA, IgG1, IgG2a, IgG2b, IgG3, and IgG4 levels when compared to healthy newborns. Additionally, differences in metabolite contents were also observed. N, N-DIMETHYLARGININE showed negative correlations with TBA, IgG1, IgG2a, IgG2b, IgG3, and IgG4, while 2-HYDROXYBUTYRATE, AMINOISOBUTANOATE, Inosine, and ALLYL ISOTHIOCYANATE exhibited positive correlations with TBA, IgG1, IgG2a, IgG2b, IgG3, and IgG4. Through metabolomics-based research, we have discovered associations between differential metabolites and different IgG isoforms during the onset of HDN.
UNASSIGNED: These findings suggest that changes in metabolite and IgG isoform levels are linked to HDN. Understanding the involvement of IgG isoforms and metabolites can provide valuable guidance for the diagnosis and treatment of HDN.
摘要：
■新生儿溶血病（HDN）是一种常见病，由于其引发的溶血反应，会对新生儿的健康产生严重影响。尽管许多研究都集中在理解HDN的发病机制上，还有许多悬而未决的问题。
■在这项回顾性研究中，收集了15名健康新生儿和8名被诊断为溶血性疾病的婴儿的血清样本。健康人群中不同代谢产物与各种IgG亚型的关系,通过生化技术和酶联免疫吸附测定（ELISA）研究HDN和BLI组。进行代谢组学分析以鉴定与HDN相关的差异代谢物。随后，使用Pearson的相关性分析来确定这些差异代谢物与IgG亚型的关系。治疗后观察代谢产物与IgG亚型的关系。
■研究结果表明，患有溶血病的婴儿表现出TBA异常升高，IgG1,IgG2a,IgG2b,与健康新生儿相比，IgG3和IgG4水平。此外,还观察到代谢物含量的差异。N,N-二甲基精氨酸与TBA呈负相关，IgG1,IgG2a,IgG2b,IgG3和IgG4，而2-羟基丁酸，氨基丁酸,肌苷,异硫氰酸烯丙基酯与TBA呈正相关，IgG1,IgG2a,IgG2b,IgG3和IgG4。通过基于代谢组学的研究，我们发现HDN发病过程中不同代谢物和不同IgG亚型之间存在关联.
■这些发现表明代谢物和IgG同种型水平的变化与HDN有关。了解IgG亚型和代谢物的参与可以为HDN的诊断和治疗提供有价值的指导。
■在这项回顾性研究中，收集了15名健康新生儿和8名被诊断为溶血性疾病的婴儿的血清样本。健康人群中不同代谢产物与各种IgG亚型的关系,通过生化技术和酶联免疫吸附测定（ELISA）研究HDN和BLI组。进行代谢组学分析以鉴定与HDN相关的差异代谢物。随后，使用Pearson的相关性分析来确定这些差异代谢物与IgG亚型的关系。治疗后观察代谢产物与IgG亚型的关系。
■研究结果表明，患有溶血病的婴儿表现出TBA异常升高，IgG1,IgG2a,IgG2b,与健康新生儿相比，IgG3和IgG4水平。此外,还观察到代谢物含量的差异。N,N-二甲基精氨酸与TBA呈负相关，IgG1,IgG2a,IgG2b,IgG3和IgG4，而2-羟基丁酸，氨基丁酸,肌苷,异硫氰酸烯丙基酯与TBA呈正相关，IgG1,IgG2a,IgG2b,IgG3和IgG4。通过基于代谢组学的研究，我们发现HDN发病过程中不同代谢物和不同IgG亚型之间存在关联.
■这些发现表明代谢物和IgG同种型水平的变化与HDN有关。了解IgG亚型和代谢物的参与可以为HDN的诊断和治疗提供有价值的指导。
