PDF(Pubmed)
Abstract：
The revolutionary technology of CRISPR/Cas has reshaped the landscape of molecular biology and molecular engineering. This tool is of interest to researchers in multiple fields, including molecular diagnostics, molecular biochemistry circuits, and information storage. As CRISPR/Cas spreads to more niche areas, new application scenarios and requirements emerge. Developing programmability and compatibility of CRISPR/Cas becomes a critical issue in the new phase. Here, we report a redundancy-based modular CRISPR/Cas12a synergistic activation platform (MCSAP). The position, length, and concentration of the redundancy in the split DNA activators can finely regulate the activity of Cas12a. With the redundant structure as an interface, MCSAP serves as a modular plug-in to seamlessly integrate with the upstream molecular network. MCSAP successfully performs three different tasks: nucleic acid detection, enzyme detection, and logic operation. MCSAP can work as an effector for different molecular networks because of its compatibility and programmability. Our platform provides powerful yet easy-to-use tools and strategies for the fields of DNA nanotechnology, molecular engineering, and molecular biology.
摘要：
CRISPR/Cas的革命性技术重塑了分子生物学和分子工程的格局。这个工具是感兴趣的研究人员在多个领域，包括分子诊断，分子生物化学回路，和信息存储。随着CRISPR/Cas传播到更多的利基领域，新的应用场景和需求出现。开发CRISPR/Cas的可编程性和兼容性成为新阶段的关键问题。这里,我们报道了一个基于冗余的模块化CRISPR/Cas12a协同激活平台(MCSAP).位置,长度,分裂DNA激活剂中冗余的浓度可以很好地调节Cas12a的活性。以冗余结构作为接口，MCSAP用作模块化插件，可与上游分子网络无缝集成。MCSAP成功执行三个不同的任务：核酸检测，酶检测，和逻辑运算。由于其兼容性和可编程性,MCSAP可以用作不同分子网络的效应物。我们的平台为DNA纳米技术领域提供了强大而易于使用的工具和策略，分子工程,和分子生物学。
