PDF(Pubmed)
Abstract：
UNASSIGNED: The clinical manifestations of pyogenic liver abscess (PLA) vary between patients with and without diabetes mellitus (DM). However, the relationship between PLA and the gut microbiome remains unknown. This study analyzed the composition of gut microbiota in PLA patients with and without DM and healthy controls (HCs) with the goal of identifying potential reasons for the observed variations in clinical manifestations.
UNASSIGNED: Using 16S ribosomal RNA(16S rRNA) gene sequencing, we analyzed the compositions of gut microbiota in 32 PLA patients with DM, 32 PLA patients without DM, and 29 matched HCs.
UNASSIGNED: In PLA patients with DM, the D-dimer level, fibrinogen degradation products, and thrombin time were significantly higher compared to the PLA patients without DM (P < 0.05). The abundance and diversity of intestinal flora were reduced in both groups of PLA patients compared with the HCs (P < 0.05). Specifically, the PLA patients with DM showed significant decreases in the relative abundances of Bacteroides, Blautia, Prevotella9, and Faecalibacterium, whereas Enterococcus and Escherichia-Shigella were relatively more abundant (P < 0.05). Compared to PLA patients without DM, those with DM had lower relative abundances of Lactobacillus and Klebsiella (P < 0.05) and showed different bacterial flora, including Anaerosporobacter and Megamonas.
UNASSIGNED: PLA patients with DM exhibited more severe clinical manifestations of PLA compared to patients without DM. It is important to monitor blood coagulation in PLA patients with DM to prevent the development of thrombotic diseases. Additionally, PLA patients with DM exhibit distinct differences in the composition and diversity of their intestinal flora compared to both PLA patients without DM and HCs.
UNASSIGNED: Using 16S ribosomal RNA(16S rRNA) gene sequencing, we analyzed the compositions of gut microbiota in 32 PLA patients with DM, 32 PLA patients without DM, and 29 matched HCs.
UNASSIGNED: In PLA patients with DM, the D-dimer level, fibrinogen degradation products, and thrombin time were significantly higher compared to the PLA patients without DM (P < 0.05). The abundance and diversity of intestinal flora were reduced in both groups of PLA patients compared with the HCs (P < 0.05). Specifically, the PLA patients with DM showed significant decreases in the relative abundances of Bacteroides, Blautia, Prevotella9, and Faecalibacterium, whereas Enterococcus and Escherichia-Shigella were relatively more abundant (P < 0.05). Compared to PLA patients without DM, those with DM had lower relative abundances of Lactobacillus and Klebsiella (P < 0.05) and showed different bacterial flora, including Anaerosporobacter and Megamonas.
UNASSIGNED: PLA patients with DM exhibited more severe clinical manifestations of PLA compared to patients without DM. It is important to monitor blood coagulation in PLA patients with DM to prevent the development of thrombotic diseases. Additionally, PLA patients with DM exhibit distinct differences in the composition and diversity of their intestinal flora compared to both PLA patients without DM and HCs.
摘要：
■化脓性肝脓肿（PLA）的临床表现在有和没有糖尿病（DM）的患者之间有所不同。然而,PLA与肠道微生物组之间的关系仍然未知。这项研究分析了患有和不患有DM和健康对照（HCs）的PLA患者的肠道菌群组成，目的是确定观察到的临床表现变化的潜在原因。
■使用16S核糖体RNA（16SrRNA）基因测序，我们分析了32名PLADM患者的肠道菌群组成，32名没有DM的PLA患者，和29个匹配的HC。
■在解放军DM患者中，D-二聚体水平,纤维蛋白原降解产物，与无DM的PLA患者相比，凝血酶时间明显升高（P<0.05）。两组患者肠道菌群的丰度和多样性均低于对照组(P<0.05)。具体来说,患有DM的PLA患者显示拟杆菌的相对丰度显着降低，Blautia,Prevotella9和Faecalibacterium，肠球菌和大肠埃希氏菌相对较丰富(P<0.05)。与没有DM的PLA患者相比，糖尿病患者乳杆菌和克雷伯菌的相对丰度较低（P<0.05），并且表现出不同的细菌菌群，包括厌氧菌和巨单胞菌.
■与没有DM的患者相比，患有DM的PLA患者表现出更严重的PLA临床表现。监测PLADM患者的凝血功能对预防血栓性疾病的发展具有重要意义。此外,与没有DM和HC的PLA患者相比，患有DM的PLA患者在其肠道菌群的组成和多样性方面表现出明显的差异。
■使用16S核糖体RNA（16SrRNA）基因测序，我们分析了32名PLADM患者的肠道菌群组成，32名没有DM的PLA患者，和29个匹配的HC。
■在解放军DM患者中，D-二聚体水平,纤维蛋白原降解产物，与无DM的PLA患者相比，凝血酶时间明显升高（P<0.05）。两组患者肠道菌群的丰度和多样性均低于对照组(P<0.05)。具体来说,患有DM的PLA患者显示拟杆菌的相对丰度显着降低，Blautia,Prevotella9和Faecalibacterium，肠球菌和大肠埃希氏菌相对较丰富(P<0.05)。与没有DM的PLA患者相比，糖尿病患者乳杆菌和克雷伯菌的相对丰度较低（P<0.05），并且表现出不同的细菌菌群，包括厌氧菌和巨单胞菌.
■与没有DM的患者相比，患有DM的PLA患者表现出更严重的PLA临床表现。监测PLADM患者的凝血功能对预防血栓性疾病的发展具有重要意义。此外,与没有DM和HC的PLA患者相比，患有DM的PLA患者在其肠道菌群的组成和多样性方面表现出明显的差异。
