PDF(Pubmed)
Abstract：
The application of enzymes as antifoulants is one of the environment-friendly strategies in biofouling management. In this study, antifouling activities of commercially available proteinase K and α-amylase enzymes were evaluated using barnacle larva and biofilm-forming bacteria as test organisms. The enzymes were also tested against barnacle cement protein through in silico analysis. The results showed that both enzymes inhibited the attachment of bacteria and settlement of barnacle larvae on the test surface. The lowest minimum inhibitory concentration of 0.312 mg ml-1 was exhibited by proteinase K against biofilm-forming bacteria. The calculated LC50 values for proteinase K and α-amylase against the barnacle nauplii were 91.8 and 230.96 mg ml-1 respectively. While α-amylase showed higher antibiofilm activity, proteinase K exhibited higher anti-larval settlement activity. Similarly, in silico analysis of the enzymes revealed promising anti-settlement activity, as the enzymes showed good binding scores with barnacle cement protein. Overall, the results suggested that the enzymes proteinase K and α-amylase could be used in antifouling coatings to reduce the settlement of biofouling on artificial materials in the marine environment.
摘要：
酶作为防污剂的应用是生物污染管理中的环境友好策略之一。在这项研究中,使用藤壶幼虫和生物膜形成细菌作为测试生物，评估了市售蛋白酶K和α-淀粉酶的防污活性。还通过硅片分析对藤壶水泥蛋白进行了酶的测试。结果表明，两种酶均能抑制细菌附着和藤壶幼虫在试验表面的沉降。蛋白酶K对生物膜形成细菌的最低最低抑制浓度为0.312mgml-1。蛋白酶K和α-淀粉酶对藤壶无节幼体的LC50计算值分别为91.8和230.96mgml-1。而α-淀粉酶显示出较高的抗生物膜活性，蛋白酶K表现出较高的抗幼虫沉降活性。同样，酶的计算机模拟分析揭示了有希望的抗沉降活性，因为酶与藤壶水泥蛋白显示出良好的结合分数。总的来说,结果表明，蛋白酶K和α-淀粉酶可用于海洋环境中的防污涂料，以减少生物污损在人工材料上的沉降。
