PDF(Pubmed)
Abstract：
UNASSIGNED: JIA is a disease with different immunological characteristics and a complicated genetic foundation. HLA B27 is a risk factor for the development of JIA, and its impact on immunopathogenesis of the disease is also an area of interest. To determine whether HLA B27 and immune markers varied between JIA patients and healthy population.
UNASSIGNED: This comparative cross-sectional study was conducted at Immunology Department of University of Health sciences (UHS), Lahore from February 2018 till August 2021. A total of (71) JIA patients and (34) healthy controls were enrolled. B cells were enumerated by flowcytometry, ELISA was used for serum cytokines estimation and HLA B27 allele was detected by SPSS polymerase chain reaction.
UNASSIGNED: The HLA B27 allele was significantly more in the control group than in the patient group, suggesting it is a protective allele to prevent JIA. Peripheral blood B cell counts and percentages were significantly lower in the HLA B27 positive group than in the HLA B27 negative group of control population. Serum cytokine levels were not significantly different between the HLA B27 positive and HLA B27 negative allele of the two study populations.
UNASSIGNED: In this study B cells are different between the two groups of control population however; serum cytokines are comparable between the study groups. Though, it was indicated that HLA B27 may be a preventive allele in the onset of JIA.
UNASSIGNED: This comparative cross-sectional study was conducted at Immunology Department of University of Health sciences (UHS), Lahore from February 2018 till August 2021. A total of (71) JIA patients and (34) healthy controls were enrolled. B cells were enumerated by flowcytometry, ELISA was used for serum cytokines estimation and HLA B27 allele was detected by SPSS polymerase chain reaction.
UNASSIGNED: The HLA B27 allele was significantly more in the control group than in the patient group, suggesting it is a protective allele to prevent JIA. Peripheral blood B cell counts and percentages were significantly lower in the HLA B27 positive group than in the HLA B27 negative group of control population. Serum cytokine levels were not significantly different between the HLA B27 positive and HLA B27 negative allele of the two study populations.
UNASSIGNED: In this study B cells are different between the two groups of control population however; serum cytokines are comparable between the study groups. Though, it was indicated that HLA B27 may be a preventive allele in the onset of JIA.
摘要：
■JIA是一种具有不同免疫学特征和复杂遗传基础的疾病。HLAB27是JIA发展的危险因素，其对该疾病的免疫发病机制的影响也是一个感兴趣的领域。确定JIA患者和健康人群之间的HLAB27和免疫标志物是否不同。
■这项比较横断面研究是在健康科学大学（UHS）免疫学系进行的，拉合尔从2018年2月到2021年8月。共纳入（71）JIA患者和（34）健康对照。通过流式细胞术计数B细胞,ELISA法检测血清细胞因子水平,SPSS聚合酶链反应法检测HLA-B27等位基因。
■对照组的HLAB27等位基因明显高于患者组，表明它是一个保护等位基因来阻止JIA.HLAB27阳性组的外周血B细胞计数和百分比显着低于对照组的HLAB27阴性组。两个研究人群的HLAB27阳性和HLAB27阴性等位基因之间的血清细胞因子水平没有显着差异。
在本研究中,B细胞在两组对照群体之间是不同的;血清细胞因子在研究组之间是相当的。不过,提示HLAB27可能是JIA发病的预防性等位基因.
■这项比较横断面研究是在健康科学大学（UHS）免疫学系进行的，拉合尔从2018年2月到2021年8月。共纳入（71）JIA患者和（34）健康对照。通过流式细胞术计数B细胞,ELISA法检测血清细胞因子水平,SPSS聚合酶链反应法检测HLA-B27等位基因。
■对照组的HLAB27等位基因明显高于患者组，表明它是一个保护等位基因来阻止JIA.HLAB27阳性组的外周血B细胞计数和百分比显着低于对照组的HLAB27阴性组。两个研究人群的HLAB27阳性和HLAB27阴性等位基因之间的血清细胞因子水平没有显着差异。
在本研究中,B细胞在两组对照群体之间是不同的;血清细胞因子在研究组之间是相当的。不过,提示HLAB27可能是JIA发病的预防性等位基因.
