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Abstract：
UNASSIGNED: This work explored the characteristics of the WRKY transcription factor family in Rhododendron henanense subsp. lingbaoense (Rhl) and the expression patterns of these genes under abiotic stress by conducting bioinformatics and expression analyses.
UNASSIGNED: RhlWRKY genes were identified from a gene library of Rhl. Various aspects of these genes were analyzed, including genetic structures, conserved sequences, physicochemical properties, cis-acting elements, and chromosomal location. RNA-seq was employed to analyze gene expression in five different tissues of Rhl: roots, stems, leaves, flowers, and hypocotyls. Additionally, qRT-PCR was used to detect changes in the expression of five RhlWRKY genes under abiotic stress.
UNASSIGNED: A total of 65 RhlWRKY genes were identified and categorized into three subfamilies based on their structural characteristics: Groups I, II, and III. Group II was further divided into five subtribes, with shared similar genetic structures and conserved motifs among members of the same subtribe. The physicochemical properties of these proteins varied, but the proteins are generally predicted to be hydrophilic. Most proteins are predicted to be in the cell nucleus, and distributed across 12 chromosomes. A total of 84 cis-acting elements were discovered, with many related to responses to biotic stress. Among the identified RhlWRKY genes, there were eight tandem duplicates and 97 segmental duplicates. The majority of duplicate gene pairs exhibited Ka/Ks values <1, indicating purification under environmental pressure. GO annotation analysis indicated that WRKY genes regulate biological processes and participate in a variety of molecular functions. Transcriptome data revealed varying expression levels of 66.15% of WRKY family genes in all five tissue types (roots, stems, leaves, flowers, and hypocotyls). Five RhlWRKY genes were selected for further characterization and there were changes in expression levels for these genes in response to various stresses.
UNASSIGNED: The analysis identified 65 RhlWRKY genes, among which the expression of WRKY_42 and WRKY_17 were mainly modulated by the drought and MeJA, and WRKY_19 was regulated by the low-temperature and high-salinity conditions. This insight into the potential functions of certain genes contributes to understanding the growth regulatory capabilities of Rhl.
UNASSIGNED: RhlWRKY genes were identified from a gene library of Rhl. Various aspects of these genes were analyzed, including genetic structures, conserved sequences, physicochemical properties, cis-acting elements, and chromosomal location. RNA-seq was employed to analyze gene expression in five different tissues of Rhl: roots, stems, leaves, flowers, and hypocotyls. Additionally, qRT-PCR was used to detect changes in the expression of five RhlWRKY genes under abiotic stress.
UNASSIGNED: A total of 65 RhlWRKY genes were identified and categorized into three subfamilies based on their structural characteristics: Groups I, II, and III. Group II was further divided into five subtribes, with shared similar genetic structures and conserved motifs among members of the same subtribe. The physicochemical properties of these proteins varied, but the proteins are generally predicted to be hydrophilic. Most proteins are predicted to be in the cell nucleus, and distributed across 12 chromosomes. A total of 84 cis-acting elements were discovered, with many related to responses to biotic stress. Among the identified RhlWRKY genes, there were eight tandem duplicates and 97 segmental duplicates. The majority of duplicate gene pairs exhibited Ka/Ks values <1, indicating purification under environmental pressure. GO annotation analysis indicated that WRKY genes regulate biological processes and participate in a variety of molecular functions. Transcriptome data revealed varying expression levels of 66.15% of WRKY family genes in all five tissue types (roots, stems, leaves, flowers, and hypocotyls). Five RhlWRKY genes were selected for further characterization and there were changes in expression levels for these genes in response to various stresses.
UNASSIGNED: The analysis identified 65 RhlWRKY genes, among which the expression of WRKY_42 and WRKY_17 were mainly modulated by the drought and MeJA, and WRKY_19 was regulated by the low-temperature and high-salinity conditions. This insight into the potential functions of certain genes contributes to understanding the growth regulatory capabilities of Rhl.
摘要：
■这项工作探索了杜鹃花亚种中WRKY转录因子家族的特征。通过进行生物信息学和表达分析，研究了这些基因在非生物胁迫下的表达模式。
■从Rhl的基因文库鉴定RhlWRKY基因。分析了这些基因的各个方面，包括遗传结构，保守序列,物理化学性质，顺式作用元素，和染色体位置。RNA-seq用于分析Rhl：根的五种不同组织中的基因表达，茎,叶子,鲜花,和下胚轴.此外,qRT-PCR检测5个RhlWRKY基因在非生物胁迫下的表达变化。
■总共鉴定了65个RhlWRKY基因，并根据其结构特征分为三个亚家族：I组，II,和III。第二组进一步分为五个部落，在同一亚部落的成员之间具有相似的遗传结构和保守的基序。这些蛋白质的物理化学性质各不相同，但是蛋白质通常被预测是亲水的。大多数蛋白质被预测在细胞核中，分布在12条染色体上.共发现84个顺式作用元件,许多与对生物应激的反应有关。在确定的RhlWRKY基因中，有8个串联重复和97个分段重复。大多数重复基因对显示Ka/Ks值<1,表明在环境压力下纯化。GO注释分析表明，WRKY基因调控生物过程，参与多种分子功能。转录组数据显示，在所有五种组织类型中，66.15%的WRKY家族基因表达水平不同（根，茎,叶子,鲜花,和下胚轴）。选择5个RhlWRKY基因用于进一步表征,并且响应于各种胁迫,这些基因的表达水平有变化。
■分析确定了65个RhlWRKY基因，其中WRKY_42和WRKY_17的表达主要受干旱和MeJA的调控,WRKY_19受低温和高盐度条件的调节。这种对某些基因的潜在功能的了解有助于理解Rhl的生长调节能力。
■从Rhl的基因文库鉴定RhlWRKY基因。分析了这些基因的各个方面，包括遗传结构，保守序列,物理化学性质，顺式作用元素，和染色体位置。RNA-seq用于分析Rhl：根的五种不同组织中的基因表达，茎,叶子,鲜花,和下胚轴.此外,qRT-PCR检测5个RhlWRKY基因在非生物胁迫下的表达变化。
■总共鉴定了65个RhlWRKY基因，并根据其结构特征分为三个亚家族：I组，II,和III。第二组进一步分为五个部落，在同一亚部落的成员之间具有相似的遗传结构和保守的基序。这些蛋白质的物理化学性质各不相同，但是蛋白质通常被预测是亲水的。大多数蛋白质被预测在细胞核中，分布在12条染色体上.共发现84个顺式作用元件,许多与对生物应激的反应有关。在确定的RhlWRKY基因中，有8个串联重复和97个分段重复。大多数重复基因对显示Ka/Ks值<1,表明在环境压力下纯化。GO注释分析表明，WRKY基因调控生物过程，参与多种分子功能。转录组数据显示，在所有五种组织类型中，66.15%的WRKY家族基因表达水平不同（根，茎,叶子,鲜花,和下胚轴）。选择5个RhlWRKY基因用于进一步表征,并且响应于各种胁迫,这些基因的表达水平有变化。
■分析确定了65个RhlWRKY基因，其中WRKY_42和WRKY_17的表达主要受干旱和MeJA的调控,WRKY_19受低温和高盐度条件的调节。这种对某些基因的潜在功能的了解有助于理解Rhl的生长调节能力。
