PDF(Pubmed)
Abstract：
This study investigated the dynamic responses to an acute glucose challenge following chronic almond versus cracker consumption for 8 weeks (clinicaltrials.gov ID: NCT03084003). Seventy-three young adults (age: 18-19 years, BMI: 18-41 kg/m2) participated in an 8-week randomized, controlled, parallel-arm intervention and were randomly assigned to consume either almonds (2 oz/d, n=38) or an isocaloric control snack of graham crackers (325 kcal/d, n=35) daily for 8 weeks. Twenty participants from each group underwent a 2-hour oral glucose tolerance test (oGTT) at the end of the 8-week intervention. Metabolite abundances in the oGTT serum samples were quantified using untargeted metabolomics, and targeted analyses for free PUFAs, total fatty acids, oxylipins, and endocannabinoids. Multivariate, univariate, and chemical enrichment analyses were conducted to identify significant metabolic shifts. Findings exhibit a biphasic lipid response distinguished by higher levels of unsaturated triglycerides in the earlier periods of the oGTT followed by lower levels in the latter period in the almond versus cracker group (p-value<0.05, chemical enrichment analyses). Almond (vs. cracker) consumption was also associated with higher AUC120 min of aminomalonate, and oxylipins (p-value<0.05), but lower AUC120 min of L-cystine, N-acetylmannosamine, and isoheptadecanoic acid (p-value<0.05). Additionally, the Matsuda Index in the almond group correlated with AUC120 min of CE 22:6 (r=-0.46; p-value<0.05) and 12,13 DiHOME (r=0.45; p-value<0.05). Almond consumption for 8 weeks leads to dynamic, differential shifts in response to an acute glucose challenge, marked by alterations in lipid and amino acid mediators involved in metabolic and physiological pathways.
摘要：
这项研究调查了慢性杏仁与饼干食用8周后对急性葡萄糖激发的动态反应（clinicaltrials.govID：NCT03084003）。73名年轻人（年龄：18-19岁，BMI：18-41kg/m2）参加了为期8周的随机分组，控制,平行臂干预，并随机分配食用杏仁(2盎司/天，n=38）或全麦饼干的等热量控制零食（325千卡/天，n=35），每天8周。每组20名参与者在8周干预结束时接受2小时口服葡萄糖耐量试验（oGTT）。使用非靶向代谢组学对oGTT血清样品中的代谢物丰度进行定量，以及针对免费PUFA的针对性分析，总脂肪酸，氧化脂素,和内源性大麻素。多变量,单变量，和化学富集分析进行，以确定显著的代谢变化。研究结果表现出双相脂质反应，其特征是在oGTT的早期阶段中不饱和甘油三酸酯的水平较高，而在杏仁与饼干组的后期阶段中不饱和甘油三酸酯的水平较低（p值<0.05，化学富集分析）。杏仁（vs.饼干)的消耗也与120分钟的氨基丙二酸的AUC较高相关，和氧化脂素（p值<0.05），但降低AUC120分钟的L-胱氨酸，N-乙酰甘露糖胺,和异十七烷酸(p值<0.05)。此外,杏仁组的松田指数与CE22：6（r=-0.46；p值<0.05）和12，13DiHOME（r=0.45；p值<0.05）的AUC120分钟相关。杏仁消费8周导致动态，响应急性葡萄糖挑战的差异变化，以参与代谢和生理途径的脂质和氨基酸介质的改变为标志。
