Abstract：
The effects of the surrounding environment on the bacterial composition of bioaerosol were well documented for polluted and contaminated sites. However, there is limited data on the impact of plant species, especially those that produce aromas, on bioaerosol composition at agricultural sites. Hence, the aim of this study is to evaluate the variability in bacterial communities present in bioaerosol samples collected from agricultural sites with aroma-producing crops. For this, PM2.5, PM10, and bioaerosol samples were collected from agricultural fields growing Ocimum [two varieties of O. sanctum (CIM-Aayu and CIM-Angana)] and O. kilimandscharicum (Kapoor), nearby traffic junctions and suburban areas. PM2.5 and PM10 concentrations at the agricultural site were in between the other two polluted sites. However, bioaerosol concentration was lower at agricultural sites than at other sites. The culturable bacteria Bacillus subtilis, Bacillus tequilensis, and Staphylococcus saprophyticus were more prevalent in agricultural sites than in other areas. However, the composition of non-culturable bacteria varied between sites and differed in three fields where Ocimum was cultivated. The CIM-Aayu cultivated area showed a high bacterial richness, lower Simpson and Shannon indices, and a distinctive metabolic profile. The sites CIM-Angana and CIM-Kapoor had a higher abundance of Aeromonas, while Pantoea and Pseudomonas were present at CIM-Aayu. Acinetobacter, Staphylococcus, and Bacillus were the dominant genera at the other two sites. Metabolic profiling showed that the CIM-Aayu site had a higher prevalence of pathways related to amino acid and carbohydrate metabolism and environmental information processing compared to other sites. The composition of bioaerosol among the three different Ocimum sites could be due to variations in the plant volatile and cross-feeding nature of bacterial isolates, which further needs to be explored.
摘要：
周围环境对生物气溶胶细菌组成的影响已在污染和污染场所得到充分证明。然而,关于植物物种影响的数据有限，尤其是那些产生香气的，农业场所的生物气溶胶成分。因此，这项研究的目的是评估从具有香气作物的农业场所收集的生物气溶胶样品中存在的细菌群落的变异性。为此，PM2.5，PM10和生物气溶胶样品是从种植Oculum[两个品种的O.sanctum（CIM-Aayu和CIM-Angana）]和O.kilimandscharicum（Kapoor）的农田中收集的，附近的交通路口和郊区。农业地点的PM2.5和PM10浓度介于其他两个污染地点之间。然而,农业地点的生物气溶胶浓度低于其他地点。可培养细菌枯草芽孢杆菌，特奎尔芽孢杆菌,腐生葡萄球菌在农业场所比其他地区更为普遍。然而,不可培养细菌的组成在不同地点之间有所不同，并且在培养Oculum的三个领域中有所不同。TheCIM-Aayu栽培面积显示出较高的细菌丰富度，较低的辛普森和香农指数，和独特的代谢特征。CIM-Angana和CIM-Kapoor的气单胞菌丰度较高，而泛菌和假单胞菌存在于CIM-Aayu。不动杆菌,葡萄球菌,芽孢杆菌是其他两个位点的优势属。代谢谱分析表明，与其他位点相比，CIM-Aayu位点与氨基酸和碳水化合物代谢以及环境信息处理相关的途径的患病率更高。三个不同Oculum位点之间的生物气溶胶组成可能是由于细菌分离株的植物挥发性和交叉摄食性质的变化，这需要进一步探索。
