Abstract：
The influence of locust bean gum (LBG) galactomannans (GMs) molecular weight (Mw) to assemble microparticulate systems was evaluated, and carriers for deep lung delivery were developed. A commercial batch of LBG with a mannose/galactose (M/G) ratio of 2.4 (batch 1) was used to study the influence of different microwave partial acid hydrolysis conditions on carbohydrate composition, glycosidic linkages, and aqueous solutions viscosity. The microwave treatment did not affect the composition, presenting 4-Man (36-42 %), 4,6-Man (27-35 %), and T-Gal (24-25 %) as the main glycosidic linkages. Depolymerization led to a viscosity reduction (≤0.005 Pa·s) with no major impact on polysaccharide debranching. The structural composition of the LBG galactomannans were further elucidated with sequence-specific proteins using carbohydrate microarray technologies. A second batch of LBG (M/G 3.3) was used to study the impact of GMs with different Mw on microparticle assembling, characteristics, and insulin release kinetics. The low-Mw GMs microparticles led to a faster release (20 min) than the higher-Mw (40 min) ones, impacting the release kinetics. All microparticles exhibited a safety profile to cells of the respiratory tract. However, only the higher-Mw GMs allowed the assembly of microparticles with sizes suitable for this type of administration.
摘要：
评估了刺槐豆胶（LBG）半乳甘露聚糖（GMs）分子量（Mw）对组装微粒系统的影响，并开发了用于深肺分娩的载体。使用甘露糖/半乳糖（M/G）比为2.4的LBG的商业批次（批次1）研究了不同的微波部分酸水解条件对碳水化合物组成的影响，糖苷键，和水溶液粘度。微波处理不影响组合物，4人（36-42%），4,6人（27-35％），和T-Gal(24-25%)作为主要的糖苷键。解聚导致粘度降低（≤0.005Pa·s），对多糖脱支没有重大影响。使用碳水化合物微阵列技术用序列特异性蛋白质进一步阐明了LBG半乳甘露聚糖的结构组成。使用第二批LBG（M/G3.3）研究不同Mw的GM对微粒组装的影响。特点,和胰岛素释放动力学。低MwGM微粒导致更快的释放（20分钟）比高Mw（40分钟）的释放，影响释放动力学。所有微粒对呼吸道细胞均表现出安全性。然而,只有较高Mw的GM允许组装具有适合这种类型给药的尺寸的微粒。
