Abstract：
Tormentilla erecta (L.) Raeusch is a widespread plant in Europe and Western Asia. Its rhizomes (Tormentilae rhizoma) are the main ingredient of herbal alcoholic beverages and can be used as a natural preservative in beer production. Apart from its unique taste qualities, therapeutic properties in gastrointestinal tract ailments are attributed to the tincture obtained from Tormentillae rhizoma. The presented research aimed to determine the mutual relationship between the components of Tormentillae tincture, present in popular alcoholic beverages, and intestinal epithelium (Caco-2 cell monolayers). A comprehensive qualitative and quantitative analysis of the tincture was performed, including the determination of condensed and hydrolyzable tannins as well as triterpenoids (UHPLC-DAD-MS/MS). Incubation of the tincture with Caco-2 monolayers has shown that only triterpenes pass through the monolayer, while condensed tannins are mainly bound to the monolayer surface. Ellagic acid derivatives were the only components of the Tormentillae tinctura being metabolized by cell monolayers to the compounds not previously described in the literature, which may be crucial in the treatment of intestinal diseases with inflammatory background.
摘要：
Tormentillaerecta(L.)Raeusch是一种在欧洲和西亚广泛种植的植物。其根茎（Tormentilaerhizoma）是草药酒精饮料的主要成分，可作为啤酒生产中的天然防腐剂。除了其独特的品味品质，胃肠道疾病的治疗特性归因于从根茎中获得的tin剂。提出的研究旨在确定Tormentillaetin剂成分之间的相互关系，目前在流行的酒精饮料，和肠上皮（Caco-2细胞单层）。进行了全面的定性和定量分析，包括确定缩合和可水解的单宁以及三萜类（UHPLC-DAD-MS/MS）。用Caco-2单层孵育tin剂表明，只有三萜通过单层，而缩合单宁主要与单层表面结合。鞣花酸衍生物是Tormentillaetin的唯一成分，被细胞单层代谢为文献中未描述的化合物。这在治疗具有炎症背景的肠道疾病中可能是至关重要的。
