Abstract：
The present study describes a novel antimicrobial mechanism based on Sodium Orthovanadate (SOV), an alkaline phosphatase inhibitor. Scanning electron microscopy (SEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM) were employed to examine the surface morphologies of the test organism, Escherichia coli (E. coli), during various antibacterial phases. Our results indicated that SOV kills bacteria by attacking cell wall growth and development, leaving E. coli\'s outer membrane intact. Our antimicrobial test indicated that the MIC of SOV for both E. coli and Lactococcus lactis (L. lactis) is 40 μM. A combination of quantum mechanical calculations and vibrational spectroscopy revealed that divanadate from SOV strongly coordinates with Ca2+ and Mg2+, which are the activity centers for the phosphatase that regulates bacterial cell wall synthesis. The current study is the first to propose the antibacterial mechanism caused by SOV attacking cell wall.
摘要：
本研究描述了一种基于原钒酸钠（SOV）的新型抗菌机制，碱性磷酸酶抑制剂.扫描电子显微镜（SEM），透射电子显微镜（TEM）和原子力显微镜（AFM）用于检查测试生物的表面形态，大肠杆菌(E.大肠杆菌)，在各种抗菌阶段。我们的结果表明，SOV通过攻击细胞壁的生长和发育来杀死细菌，留下大肠杆菌的外膜完整。我们的抗菌测试表明，SOV对大肠杆菌和乳酸乳球菌的MIC（L.乳酸）为40μM。量子力学计算和振动光谱的结合表明，SOV的钒酸盐与Ca2和Mg2强配位，它们是调节细菌细胞壁合成的磷酸酶的活动中心。目前的研究首次提出了SOV攻击细胞壁引起的抗菌机制。
