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Abstract：
Getah virus (GETV) is a re-emerging mosquito-borne alphavirus that is highly pathogenic, mainly to pigs and horses. There are no vaccines or treatments available for GETV in swine in China. Therefore, the development of a simple, rapid, specific, and sensitive serological assay for GETV antibodies is essential for the prevention and control of GETV. Current antibody monitoring methods are time-consuming, expensive, and dependent on specialized instrumentation, and these features are not conducive to rapid detection in clinical samples. To address these problem, we developed immunochromatographic test strips (ICTS) using eukaryotically expressed soluble recombinant p62-E1 protein of GETV as a labelled antigen, which has good detection sensitivity and no cross-reactivity with other common porcine virus-positive sera. The ICTS is highly compatible with IFA and ELISA and can be stored for 1 month at 37 °C and for at least 3 months at room temperature. Hence, p62-E1-based ICTS is a rapid, accurate, and convenient method for rapid on-site detection of GETV antibodies. KEY POINTS: • We established a rapid antibody detection method that can monitor GETV infection • We developed colloidal gold test strips with high sensitivity and specificity • The development of colloidal gold test strips will aid in the field serologic detection of GETV.
摘要：
Getah病毒（GETV）是一种重新出现的蚊媒α病毒，具有高致病性，主要是猪和马。在中国，没有可用于GETV猪的疫苗或治疗方法。因此,开发一个简单的，快速,具体,敏感的GETV抗体血清学检测对GETV的预防和控制至关重要。目前的抗体监测方法耗时,贵,依赖于专门的仪器，这些特征不利于临床样本的快速检测。为了解决这些问题,我们使用真核细胞表达的GETV的可溶性重组p62-E1蛋白作为标记抗原开发了免疫层析试纸条（ICTS），具有良好的检测灵敏度，与其他常见猪病毒阳性血清无交叉反应。ICTS与IFA和ELISA高度兼容，可以在37°C下储存1个月，在室温下储存至少3个月。因此，基于P62-E1的ICTS是一种快速，准确,快速现场检测GETV抗体的简便方法。要点：•我们建立了一种可以监测GETV感染的快速抗体检测方法•我们开发了具有高灵敏度和特异性的胶体金测试条•胶体金测试条的开发将有助于GETV的现场血清学检测。
