Abstract：
Purpose: The purposes of this in vitro study were to evaluate the effect of three isolation methods to mitigate bioaerosols during stainless steel crown (SSC) preparations and assess the distribution of Streptococcus mutans by aerosolization in closed-room operatories. Methods: Melamine teeth coated in laboratory-grown S. mutans biofilm were prepared for SSCs using three different isolation methods. Agar plates were placed in five locations throughout the operatory and opened during each preparation as well as for 10 minutes immediately following to collect aerosolized S. mutans. Bacterial colonies were counted after incubating plates for 48 hours. Data were analyzed for differences between the isolation method and plate locations. Results: Bacterial colony counts for teeth prepared using high-volume evacuation suction (HVE) with dental dam (DD) isolation were statistically significantly higher than for those prepared using HVE with a DryShield®(DS) and HVE with no isolation at the assistant (A) (P<0.001), operator face shield (FS) (P<0.001), and patient (Pt) (P=0.002) locations. No significant differences were found among isolation methods for parent (Pa) or rear delivery (RD) locations. The location that produced the most bacterial colony counts using HVE with DD isolation was FS (P<0.001), followed by A (P=0.04), Pt (P<0.001), and RD and Pa (P<0.001). Counts produced from teeth prepared with DS isolation were significantly higher at the Pt location than the A (P<0.001), FS (P=0.002), RD (P<0.001), and Pa (P=0.008) locations. Conclusion: The use of dental dam with high-volume evacuation suction during stainless steel crown preparations increased bioaerosols near the procedure, while dental evacuation systems (DryShield®) may effectively limit their spread.
摘要：
目的：这项体外研究的目的是评估三种分离方法在不锈钢冠（SSC）制备过程中减轻生物气溶胶的效果，并通过密闭手术室中的雾化来评估变形链球菌的分布。方法：使用三种不同的分离方法为SSCs制备了在实验室生长的变形链球菌生物膜中涂覆的三聚氰胺牙齿。在整个手术过程中,将琼脂板放置在五个位置,并在每次制备过程中以及在收集雾化变形链球菌后立即打开10分钟。在培养板48小时后计数细菌菌落。分析了隔离方法和板位置之间的差异。结果：使用具有牙坝（DD）隔离的高容量排空吸引（HVE）制备的牙齿的细菌菌落计数在统计学上显着高于使用具有DryShield®（DS）的HVE和在助手（A）没有隔离的HVE（P<0.001），操作员面罩（FS）（P<0.001），和患者（Pt）（P=0.002）位置。在父母（Pa）或后方分娩（RD）位置的隔离方法之间没有发现显着差异。使用DD分离的HVE产生最多的细菌菌落计数的位置是FS（P<0.001），其次是A（P=0.04），Pt（P<0.001），RD和Pa(P<0.001)。从DS分离制备的牙齿产生的计数在Pt位置明显高于A（P<0.001），FS（P=0.002），RD（P<0.001），和Pa(P=0.008)位置。结论：在不锈钢牙冠制备过程中，使用具有高容量排空吸引的牙坝会增加手术附近的生物气溶胶，而牙科疏散系统（DryShield®）可以有效地限制其传播。
