Abstract：
Recent metanalysis reported that certain probiotic strains, such as Limosilactobacillus reuteri and Lacticaseibacillus rhamnosus (LGG), seem effective for treatment of infantile colic of exclusively breastfed infants; some reports have also linked probiotics to have an immunological effect, however further investigation are needed to fully understand the exact mechanism. The objective of this study was to assay white blood cells, tumour necrosis factor (TNF)-α and interleukin (IL)-6 values in peripheral blood in subjects treated in a randomised, double-blind, placebo-controlled trial for infantile colic with LGG. Fifty-eight infants were enrolled and followed for a study period of 28 days. Parent were asked to record daily crying time using a structured cry diary. Peripheral white blood cells was assessed and RNA (mRNA) expression of TNF-α and IL-6 was measured using TaqMan real-time PCR-maternal amplification. Infants with colic treated with LGG showed a reduction in daily crying duration after 28 days of treatment and a reduction in values of IL-6 ( P < 0.005) and TNF-α ( P < 0.05); we observe also a significantly decreasing of IL-6 in the placebo group while decrease of TNF-α was not significant in this group. A significant decreased values of monocytes ( P < 0.05) was observed in infants treated with LGG. Our data therefore showed, in addition to crying time reduction, a significant decrease of TNF-α and a significant reduction of monocytes cells in colicky infants treated with LGG, compared to placebo group. This observation supports the hypothesis that probiotics may have anti-inflammatory properties. Further studies are needed to better understand the influence of probiotic on immunity cells.
摘要：
最近的荟萃分析报告说，某些益生菌菌株，如罗伊利烟杆菌和鼠李糖乳杆菌(LGG)，似乎对治疗纯母乳喂养婴儿的婴儿绞痛有效;一些报道还将益生菌与免疫效果联系起来，然而，需要进一步调查才能充分了解确切的机制。这项研究的目的是测定白细胞，肿瘤坏死因子（TNF）-α和白细胞介素（IL）-6值在一个随机的治疗对象，双盲,LGG婴儿绞痛的安慰剂对照试验。招募了58名婴儿，并随访了28天的研究期。要求父母使用结构化的哭泣日记记录每天的哭泣时间。使用TaqMan实时PCR-母体扩增评估外周血白细胞，并测量TNF-α和IL-6的RNA（mRNA）表达。用LGG治疗的绞痛婴儿在治疗28天后显示每日哭泣持续时间减少，IL-6（P<0.005）和TNF-α（P<0.05）的值降低；我们还观察到安慰剂组中IL-6的显着降低，而该组中TNF-α的降低不明显。在用LGG治疗的婴儿中观察到单核细胞值的显著降低(P<0.05)。因此，我们的数据显示，除了哭泣时间减少，在接受LGG治疗的绞痛婴儿中，TNF-α的显着降低和单核细胞的显着减少，与安慰剂组相比。这一观察结果支持益生菌可能具有抗炎特性的假设。需要进一步的研究来更好地了解益生菌对免疫细胞的影响。
