Abstract：
Plastic polymers were largely added with chemical substances to be utilized in the items and product manufacturing. The leachability of these substances is a matter of concern given the wide amount of plastic waste, particularly in terrestrial environments, where soil represents a sink for these novel contaminants and a possible pathway of human health risk. In this study, we integrated genetic, molecular, and behavioral approaches to comparatively evaluate toxicological effects of plastic leachates, virgin and oxodegradable polypropylene (PP) and polyethylene (PE), in Drosophila melanogaster, a novel in vivo model organism for environmental monitoring studies and (eco)toxicological research. The results of this study revealed that while conventional toxicological endpoints such as developmental times and longevity remain largely unaffected, exposure to plastic leachates induces chromosomal abnormalities and transposable element (TE) activation in neural tissues. The combined effects of DNA damage and TE mobilization contribute to genome instability and increase the likelihood of LOH events, thus potentiating tumor growth and metastatic behavior ofRasV12 clones. Collectively, these findings indicate that plastic leachates exert genotoxic effects in Drosophila thus highlighting potential risks associated with leachate-related plastic pollution and their implications for ecosystems and human health.
摘要：
塑料聚合物大量添加化学物质以用于物品和产品制造。鉴于大量的塑料废物，这些物质的可浸出性是一个值得关注的问题，特别是在陆地环境中，土壤代表了这些新型污染物的汇和人类健康风险的可能途径。在这项研究中,我们整合了遗传，分子，以及比较评估塑料渗滤液毒理学影响的行为方法，原始和可氧化降解的聚丙烯（PP）和聚乙烯（PE），在黑腹果蝇中，一种用于环境监测研究和（生态）毒理学研究的新型体内模型生物。这项研究的结果表明，尽管发育时间和寿命等常规毒理学终点在很大程度上不受影响，暴露于塑料渗滤液会诱导神经组织中的染色体异常和转座因子（TE）激活。DNA损伤和TE动员的联合作用有助于基因组不稳定并增加LOH事件的可能性，从而增强RasV12克隆的肿瘤生长和转移行为。总的来说，这些发现表明，塑料渗滤液在果蝇中发挥基因毒性作用，从而突出了与渗滤液相关的塑料污染相关的潜在风险及其对生态系统和人类健康的影响。
