PDF(Pubmed)
Abstract：
BACKGROUND: Koi herpesvirus disease (KHVD) is attributed to cyprinid herpesvirus-3 (CyHV-3) and predominantly affects common carp and ornamental koi carp (Cyprinus carpio). This viral infection leads to substantial morbidity and mortality among these fish species. This study aimed to confirm the presence of KHVD in the Kurdistan region of Iraq by employing clinical and optimized molecular assays on fish populations experiencing high mortality among common carp in carp farms.
METHODS: The present research was conducted in the Kalar district, situated at the heart of Garmian province in Iraqi Kurdistan. four samples from common carp fish farms were received by our laboratory. These samples specifically displaying clinical signs associated with koi herpesvirus (KHV) infection, were subjected to clinical examinations, and PCR assay in addition to sequence analysis.
RESULTS: The results of the current study revealed that the observed clinical signs, particularly gill necrosis, skin lesions, and sunken eyes, closely resembled the clinical signs of KHVD in common carp fish. In addition, PCR, nested PCR, and sequence analysis assay detected appropriate DNA fragments of the CyHV-3 major capsid protein gene confirming the first detection of KHVD in common carp fish in the Kurdistan region of Iraq.
CONCLUSIONS: In this study, the results confirm the detection of KHVD in the Kurdistan region, Iraq, for the first time. This study revealed that CyHV-3 was responsible for KHVD-related signs and symptoms. Based on these results, it is strongly recommended that comprehensive studies be initiated to investigate the prevalence and distribution of CyHV-3.
METHODS: The present research was conducted in the Kalar district, situated at the heart of Garmian province in Iraqi Kurdistan. four samples from common carp fish farms were received by our laboratory. These samples specifically displaying clinical signs associated with koi herpesvirus (KHV) infection, were subjected to clinical examinations, and PCR assay in addition to sequence analysis.
RESULTS: The results of the current study revealed that the observed clinical signs, particularly gill necrosis, skin lesions, and sunken eyes, closely resembled the clinical signs of KHVD in common carp fish. In addition, PCR, nested PCR, and sequence analysis assay detected appropriate DNA fragments of the CyHV-3 major capsid protein gene confirming the first detection of KHVD in common carp fish in the Kurdistan region of Iraq.
CONCLUSIONS: In this study, the results confirm the detection of KHVD in the Kurdistan region, Iraq, for the first time. This study revealed that CyHV-3 was responsible for KHVD-related signs and symptoms. Based on these results, it is strongly recommended that comprehensive studies be initiated to investigate the prevalence and distribution of CyHV-3.
摘要：
背景：锦鱼疱疹病毒病（KHVD）归因于鲤鱼疱疹病毒3（CyHV-3），主要影响鲤鱼和观赏鱼（Cyprinuscarpio）。这种病毒感染导致这些鱼类的大量发病率和死亡率。这项研究旨在通过对鲤鱼养殖场中鲤鱼死亡率高的鱼类种群进行临床和优化的分子测定，来确认伊拉克库尔德斯坦地区KHVD的存在。
方法：本研究是在卡拉地区进行的，位于伊拉克库尔德斯坦加米安省的中心。我们的实验室收到了来自鲤鱼养殖场的四个样本。这些样本特别显示与锦松疱疹病毒（KHV）感染相关的临床症状，接受了临床检查，和PCR分析以及序列分析。
结果：当前研究的结果表明，观察到的临床体征，尤其是ill坏死，皮肤损伤,凹陷的眼睛，与鲤鱼中KHVD的临床症状非常相似。此外,PCR,巢式PCR，和序列分析测定检测到CyHV-3主要衣壳蛋白基因的适当DNA片段，证实了在伊拉克库尔德斯坦地区的鲤鱼鱼中首次检测到KHVD。
结论：在这项研究中，结果证实了在库尔德斯坦地区检测到KHVD，伊拉克,第一次。这项研究表明，CyHV-3是与KHVD相关的体征和症状的原因。基于这些结果,强烈建议启动全面研究,以调查CyHV-3的患病率和分布.
方法：本研究是在卡拉地区进行的，位于伊拉克库尔德斯坦加米安省的中心。我们的实验室收到了来自鲤鱼养殖场的四个样本。这些样本特别显示与锦松疱疹病毒（KHV）感染相关的临床症状，接受了临床检查，和PCR分析以及序列分析。
结果：当前研究的结果表明，观察到的临床体征，尤其是ill坏死，皮肤损伤,凹陷的眼睛，与鲤鱼中KHVD的临床症状非常相似。此外,PCR,巢式PCR，和序列分析测定检测到CyHV-3主要衣壳蛋白基因的适当DNA片段，证实了在伊拉克库尔德斯坦地区的鲤鱼鱼中首次检测到KHVD。
结论：在这项研究中，结果证实了在库尔德斯坦地区检测到KHVD，伊拉克,第一次。这项研究表明，CyHV-3是与KHVD相关的体征和症状的原因。基于这些结果,强烈建议启动全面研究,以调查CyHV-3的患病率和分布.
