Abstract：
The objective of this study was to investigate the presence and genetic attributes of Borrelia spp. in cats and dogs from the West Azerbaijan Province, located in the northwest of Iran. A total of 250 blood samples from cats and 300 blood samples from dogs were collected, and information regarding their age, sex, breed, ownership status, sampling time and region was recorded. The identification of positive samples was accomplished through nested-PCR and sequencing, with subsequent analysis of the gene sequences conducted using BioEdit software. The gene sequences for Borrelia spp. in this study showed 100% similarity to reference sequences in the GenBank® database. Phylogenetic trees were built using MEGA11. The outcomes indicated that among 250 blood samples from cats, 48 (19.2%) tested positive for Borrelia spp. gene, with a CI from 14.8 to 24.53% for cats. Similarly, out of 300 blood samples from dogs, 45 (15%) tested positive for the Borrelia spp. gene, with a CI from 11.4 to 19.48% for dogs.
摘要：
这项研究的目的是调查疏螺旋体属的存在和遗传属性。来自西阿塞拜疆省的猫和狗，位于伊朗西北部。共收集了猫的250份血样和狗的300份血样,以及关于他们年龄的信息，性别,品种,所有权状态，记录采样时间和区域。通过巢式PCR和测序完成阳性样品的鉴定，随后使用BioEdit软件对基因序列进行分析。疏螺旋体属的基因序列。在这项研究中，与GenBank®数据库中的参考序列具有100%的相似性。使用MEGA11构建系统发育树。结果表明，在猫的250份血液样本中，48（19.2％）的疏螺旋体属检测呈阳性。基因，猫的aCI从14.8%到24.53%。同样，在狗的300份血样中,45（15％）的疏螺旋体属检测呈阳性。基因，狗的aCI从11.4到19.48％。
