PDF(Pubmed)
Abstract：
Electrical muscle stimulation (EMS) has been shown to stimulate the production of myokines (i.e., brain-derived neurotrophic factor (BDNF)), but the most effective EMS parameters for myokine production have not been fully elucidated. The purpose of this study was to quantify the optimal EMS frequency for stimulating myokine production. This study included sixteen young adults (male, n = 13, age = 27.3 ± 5.5 years). Participants underwent four EMS interventions (20 min each) with the following conditions: (1) 4 Hz, (2) 20 Hz, (3) 80 Hz, and (4) control (no intervention). Blood samples were obtained before and immediately after EMS. For the control condition, blood samples were taken before and after 20 min of quiet sitting. BDNF and cathepsin-B levels were analyzed in serum. Compared to preintervention levels, stimulation at 20 Hz resulted in significantly greater postintervention cathepsin-B and BDNF levels (p < 0.01). On the other hand, the control condition did not result in a significant change between pre- and posttreatment. Furthermore, stimulation at 20 Hz caused significantly larger increases in cathepsin-B and BDNF levels than stimulation at 4-80 Hz or the control condition (p < 0.05). In conclusion, stimulation at 20 Hz effectively causes a robust cathepsin-B and BDNF response. Based on these results, we suggest a new strategy for rehabilitation of people with neurological disorders.
摘要：
电肌肉刺激(EMS)已被证明可以刺激肌动蛋白的产生(即，脑源性神经营养因子（BDNF），但最有效的EMS参数用于生产Myokine尚未完全阐明。这项研究的目的是量化刺激肌细胞产生的最佳EMS频率。这项研究包括16名年轻人(男性，n=13，年龄=27.3±5.5岁）。参与者接受了四次EMS干预（每次20分钟），条件如下：（1）4Hz，(2)20Hz,(3)80Hz,（4）控制（无干预）。在EMS之前和之后立即获得血液样品。对于控制条件，静坐20分钟前后采集血样.分析血清中BDNF和组织蛋白酶B的水平。与干预前水平相比，20Hz刺激导致干预后组织蛋白酶B和BDNF水平显著升高(p<0.01)。另一方面,对照条件没有导致治疗前和治疗后之间的显著变化.此外，与4-80Hz或对照条件下的刺激相比，20Hz下的刺激引起的组织蛋白酶B和BDNF水平的增加明显更大（p<0.05）。总之,在20Hz下的刺激有效地引起强烈的组织蛋白酶-B和BDNF响应。基于这些结果,我们提出了一种新的神经系统疾病患者的康复策略。
