Abstract：
Among the MAP1LC3/LC3 subfamily of Atg8 proteins, LC3B and LC3C constitute the most and least studied members, respectively, LC3B being generally considered as an autophagosomal marker and a canonical representative of the LC3 subfamily. In several recent studies, LC3C has emerged as an important modulator in various processes of cell homeostasis. Our own research data demonstrate that LC3C induces higher levels of tethering and of intervesicular lipid mixing than LC3B. LC3C contains a peculiar N-terminal region, different from the other Atg8-family protein members. Using a series of mutants, we have shown that the N-terminal region of LC3C is responsible for the enhanced vesicle tethering, membrane perturbation and vesicle-vesicle fusion activities of LC3C as compared to LC3B.Abbreviations: ATG: autophagy related; GABARAP: gamma-aminobutyric acid receptor associated protein; MAP1LC3/LC3: microtubule-associated protein 1 light chain 3; PC: phosphatidyl choline; PE: phosphatidylethanolamine; PEmal: maleimide-derivatized PE; PtdIns: phosphatidylinositol.
摘要：
在Atg8蛋白的MAP1LC3/LC3亚家族中，LC3B和LC3C构成了研究最多和最少的成员，分别,LC3B通常被认为是自噬体标记和LC3亚家族的典型代表。在最近的几项研究中，LC3C已成为各种细胞稳态过程中的重要调节剂。我们自己的研究数据表明，LC3C比LC3B诱导更高水平的系链和囊泡间脂质混合。LC3C包含一个特殊的N端区域，与其他Atg8家族蛋白成员不同。使用一系列突变体，我们已经表明，LC3C的N端区域负责增强的囊泡连接，与LC3B相比，LC3C的膜扰动和囊泡-囊泡融合活性。缩写:ATG:自噬相关;GABARAP:γ-氨基丁酸受体相关蛋白;MAP1LC3/LC3:微管相关蛋白1轻链3;PC:磷脂酰胆碱;PE:磷脂酰乙醇胺;PEmal:马来酰亚胺化衍生物;PtdIns:磷脂酰肌醇。
