Abstract：
In microbiological research, traditional methods for bacterial screening and antibiotic susceptibility testing are resource-intensive. Microfluidics offers an efficient alternative with rapid results and minimal sample consumption, but the demand for cost-effective, user-friendly platforms persists in communities and hospitals. Inspired by the Magdeburg hemispheres, the strategy adapts to local conditions, leveraging omnipresent atmospheric pressure for self-sealing of Rotation-SlipChip (RSC) equipped with a 3D circular Christmas tree-like microfluidic concentration gradient generator. This innovative approach provides an accessible and adaptable platform for microbiological research and testing in diverse settings. The RSC can avoid leakage concerns during multiple concentration gradient generation, chip-rotating, and final long-term incubation reaction (≥24 h). Furtherly, RSC subtypes adapted to different reactions can be fabricated in less than 15 min with cost less than $1, the result can be read through designated observational windows by naked-eye. Moreover, the RSC demonstrates its capability for evaluating bacterial biomarker activity, enabling the rapid assessment of β-galactosidase concentration and enzyme activity within 30 min, and the limit of detection can be reduced by 10-fold. It also rapidly determines the minimum antibiotic inhibitory concentration and antibiotic combined medications results within 4 h. Overall, these low-cost and user-friendly RSC make them invaluable tools in determinations at previously impractical environment.
摘要：
在微生物学研究中,传统的细菌筛选和抗生素药敏试验方法是资源密集型的。微流控技术提供了一种高效的替代方案，具有快速的结果和最小的样品消耗，但是对成本效益的需求，用户友好的平台在社区和医院仍然存在。受马格德堡半球的启发，战略因地制宜,利用无所不在的大气压力进行旋转滑动芯片（RSC）的自密封，配备了3D圆形圣诞树状微流体浓度梯度发生器。这种创新的方法为不同环境中的微生物研究和测试提供了一个可访问和可适应的平台。RSC可以避免在多个浓度梯度生成期间的泄漏问题，芯片旋转，和最终的长期孵育反应（≥24小时）。更进一步,适应不同反应的RSC亚型可以在不到15分钟的时间内制造，成本低于1美元，结果可以通过指定的观察窗口通过肉眼读取。此外，RSC展示了其评估细菌生物标志物活性的能力，能够在30分钟内快速评估β-半乳糖苷酶浓度和酶活性，检测限可以降低10倍。它还可以在4小时内快速确定最小抗生素抑制浓度和抗生素联合用药结果。这些低成本和用户友好的RSC使它们成为在以前不切实际的环境中确定的宝贵工具。
