PDF(Pubmed)
Abstract：
BACKGROUND: Previous studies have validated the efficacy of both magnetic compression and surgical techniques in creating rabbit tracheoesophageal fistula (TEF) models. Magnetic compression achieves a 100% success rate but requires more time, while surgery, though less frequently successful, offers rapid model establishment and technical maturity in larger animal models.
OBJECTIVE: To determine the optimal approach for rabbit disease modeling and refine the process.
METHODS: TEF models were created in 12 rabbits using both the modified magnetic compression technique and surgery. Comparisons of the time to model establishment, success rate, food and water intake, weight changes, activity levels, bronchoscopy findings, white blood cell counts, and biopsies were performed. In response to the failures encountered during modified magnetic compression modeling, we increased the sample size to 15 rabbit models and assessed the repeatability and stability of the models, comparing them with the original magnetic compression technique.
RESULTS: The modified magnetic compression technique achieved a 66.7% success rate, whereas the success rate of the surgery technique was 33.3%. Surviving surgical rabbits might not meet subsequent experimental requirements due to TEF-related inflammation. In the modified magnetic compression group, one rabbit died, possibly due to magnet corrosion, and another died from tracheal magnet obstruction. Similar events occurred during the second round of modified magnetic compression modeling, with one rabbit possibly succumbing to aggravated lung infection. The operation time of the first round of modified magnetic compression was 3.2 ± 0.6 min, which was significantly reduced to 2.1 ± 0.4 min in the second round, compared to both the first round and that of the original technique.
CONCLUSIONS: The modified magnetic compression technique exhibits lower stress responses, a simple procedure, a high success rate, and lower modeling costs, making it a more appropriate choice for constructing TEF models in rabbits.
OBJECTIVE: To determine the optimal approach for rabbit disease modeling and refine the process.
METHODS: TEF models were created in 12 rabbits using both the modified magnetic compression technique and surgery. Comparisons of the time to model establishment, success rate, food and water intake, weight changes, activity levels, bronchoscopy findings, white blood cell counts, and biopsies were performed. In response to the failures encountered during modified magnetic compression modeling, we increased the sample size to 15 rabbit models and assessed the repeatability and stability of the models, comparing them with the original magnetic compression technique.
RESULTS: The modified magnetic compression technique achieved a 66.7% success rate, whereas the success rate of the surgery technique was 33.3%. Surviving surgical rabbits might not meet subsequent experimental requirements due to TEF-related inflammation. In the modified magnetic compression group, one rabbit died, possibly due to magnet corrosion, and another died from tracheal magnet obstruction. Similar events occurred during the second round of modified magnetic compression modeling, with one rabbit possibly succumbing to aggravated lung infection. The operation time of the first round of modified magnetic compression was 3.2 ± 0.6 min, which was significantly reduced to 2.1 ± 0.4 min in the second round, compared to both the first round and that of the original technique.
CONCLUSIONS: The modified magnetic compression technique exhibits lower stress responses, a simple procedure, a high success rate, and lower modeling costs, making it a more appropriate choice for constructing TEF models in rabbits.
摘要：
背景：先前的研究已经验证了磁压缩和手术技术在创建兔气管食管瘘（TEF）模型中的有效性。磁压缩达到100％的成功率，但需要更多的时间，手术时，虽然不太成功，在大型动物模型中提供快速模型建立和技术成熟。
目的：确定兔疾病建模的最佳方法并完善过程。
方法：使用改良的磁压缩技术和手术在12只兔子中创建TEF模型。模型建立时间的比较，成功率,食物和水的摄入，体重变化,活动水平,支气管镜检查结果，白细胞计数,并进行了活检。响应在修改的磁压缩建模过程中遇到的故障，我们将样本量增加到15只兔子模型，并评估了模型的可重复性和稳定性，将它们与原始的磁压缩技术进行比较。
结果：改进的磁压缩技术获得了66.7％的成功率，而手术技术的成功率为33.3%。由于TEF相关的炎症，存活的手术兔可能无法满足随后的实验要求。在改良的磁压缩组中，一只兔子死了,可能是由于磁铁腐蚀,还有一个人死于气管磁铁阻塞.在第二轮修改的磁压缩建模过程中也发生了类似的事件，一只兔子可能死于加重的肺部感染。第一轮改良磁压操作时间为3.2±0.6min,在第二轮中明显减少到2.1±0.4分钟，与第一轮和原始技术相比。
结论：改进的磁压缩技术表现出较低的应力响应，一个简单的程序，成功率很高,更低的建模成本，使其成为构建兔TEF模型的更合适的选择。
目的：确定兔疾病建模的最佳方法并完善过程。
方法：使用改良的磁压缩技术和手术在12只兔子中创建TEF模型。模型建立时间的比较，成功率,食物和水的摄入，体重变化,活动水平,支气管镜检查结果，白细胞计数,并进行了活检。响应在修改的磁压缩建模过程中遇到的故障，我们将样本量增加到15只兔子模型，并评估了模型的可重复性和稳定性，将它们与原始的磁压缩技术进行比较。
结果：改进的磁压缩技术获得了66.7％的成功率，而手术技术的成功率为33.3%。由于TEF相关的炎症，存活的手术兔可能无法满足随后的实验要求。在改良的磁压缩组中，一只兔子死了,可能是由于磁铁腐蚀,还有一个人死于气管磁铁阻塞.在第二轮修改的磁压缩建模过程中也发生了类似的事件，一只兔子可能死于加重的肺部感染。第一轮改良磁压操作时间为3.2±0.6min,在第二轮中明显减少到2.1±0.4分钟，与第一轮和原始技术相比。
结论：改进的磁压缩技术表现出较低的应力响应，一个简单的程序，成功率很高,更低的建模成本，使其成为构建兔TEF模型的更合适的选择。
