Mesh : Animals Genome Chromosomes Echinodermata / genetics Molecular Sequence Annotation Base Composition DNA Transposable Elements

来  源:   DOI:10.1038/s41597-024-03412-y   PDF(Pubmed)

Abstract:
The cold-water species Ophiura sarsii, a brittle star, is a key echinoderm in the Arctic continental shelf region, highly sensitive to climate change. However, the absence of a high-quality genome has hindered a thorough understanding of its adaptive evolution. In this study, we reported the first chromosome-level genome assembly of O. sarsii. The genome assembly totalled 1.57 Gb, encompassing 19 chromosomes with a GC content of 37.11% and a scaffold N50 length of 78.03 Mb. The Benchmarking Universal Single-Copy Orthologs (BUSCO) assessment yielded a completeness estimate of 93.5% for this assembly. We predicted a total of 27,099 protein-coding genes, with 25,079 functionally annotated. The genome was comprised of 58.09% transposable elements. This chromosome-level genome of O. sarsii contributes to our understanding of the origin and evolution of marine organisms.
摘要:
冷水物种Ophiurasarsii,一颗脆弱的星星,是北极大陆架地区的重要棘皮动物,对气候变化高度敏感。然而,缺乏高质量的基因组阻碍了对其适应性进化的透彻理解。在这项研究中,我们报道了O.sarsii的第一个染色体水平基因组组装。基因组组装总计1.57Gb,包含19条染色体,GC含量为37.11%,支架N50长度为78.03Mb。基准通用单拷贝直射同源物(BUSCO)评估得出该组件的完整性估计为93.5%。我们预测了总共27,099个蛋白质编码基因,带有25,079个功能注释。基因组由58.09%的转座因子组成。O.sarsii的染色体水平基因组有助于我们了解海洋生物的起源和进化。
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